Capillary electrophoresis with solid-state electrochemiluminescence detector.

Journal Article (Journal Article)

We report capillary electrophoresis coupling to a solid-state electrochemiluminescence (ECL) detector for the first time. The solid-state ECL detector was fabricated by immobilizing the ECL reagent tris(2,2'-bipyridyl)ruthenium (TBR) in poly-(p-styrenesulfonate)-silica-poly(vinyl alcohol) grafting 4-vinylpyridine copolymer films. The excellent stability of the solid-state ECL detector in the phosphate solution satisfied application in CE. The CE with solid-state ECL detector system was characterized using tripropylamine (TPA) and proline. The influences of detection potential, the concentration of TBR in the film, and pH value of ECL buffer were investigated. The linear range for TPA and proline was 0.005-10 microM and 5-10 mM with correlation coefficients of 0.997 and 0.998, respectively. The detection limit (signal-to-noise ratio S/N = 3) was estimated to be 0.002 and 2.0 microM for TPA and proline, respectively. The relative standard deviations for 1.0 microM TPA and 1.0 mM proline were 8.7% and 7.5% with theoretical plate numbers of 70 000 and 16 000, respectively. Compared with the CE-ECL of TBR in aqueous solution, the CE coupling with solid-state ECL detector system gave the same sensitivity of analysis.

Full Text

Duke Authors

Cited Authors

  • Cao, W; Jia, J; Yang, X; Dong, S; Wang, E

Published Date

  • November 2002

Published In

Volume / Issue

  • 23 / 21

Start / End Page

  • 3692 - 3698

PubMed ID

  • 12432530

International Standard Serial Number (ISSN)

  • 0173-0835

Digital Object Identifier (DOI)

  • 10.1002/1522-2683(200211)23:21<3692::AID-ELPS3692>3.0.CO;2-J


  • eng

Conference Location

  • Germany