In vivo and in vitro effects of diisopropyl phosphorofluoridate (DFP) on the rate of hen brain tubulin polymerization.


Journal Article

Diisopropyl phosphorofluoridate (DFP) produces organophosphorus ester-induced delayed neurotoxicity (OPIDN) in sensitive species. We have investigated the in vivo and in vitro effects of DFP on hen brain tubulin polymerization. Hens were treated with a single dose of DFP (1.7 mg/kg, sc.), and were sacrificed after 18-21 days. Tubulin from DFP-treated hen brains showed small but significant decrease (14.42%) in the rate of polymerization and 11.05% decrease in rise in O.D. at 340 nm in 30 min. DFP in vivo treatment also resulted in decreased concentration of tau and an enhanced concentration of two peptides (45 kDa, 35 kDa) in the brain supernatant. These peptides seemed to be the degradation products of MAP-2. The decrease in the rate of brain tubulin polymerization in treated hens is consistent with neurochemical alterations and the focal degeneration and aggregation of these filamentous structures in OPIDN.

Full Text

Duke Authors

Cited Authors

  • Gupta, RP; Abou-Donia, MB

Published Date

  • April 1, 1994

Published In

Volume / Issue

  • 19 / 4

Start / End Page

  • 435 - 444

PubMed ID

  • 8065500

Pubmed Central ID

  • 8065500

International Standard Serial Number (ISSN)

  • 0364-3190

Digital Object Identifier (DOI)

  • 10.1007/bf00967321


  • eng

Conference Location

  • United States