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The transcriptional integrator CREB-binding protein mediates positive cross talk between nuclear hormone receptors and the hematopoietic bZip protein p45/NF-E2.

Publication ,  Journal Article
Cheng, X; Reginato, MJ; Andrews, NC; Lazar, MA
Published in: Mol Cell Biol
March 1997

Thyroid hormone (T3) and retinoic acid (RA) play important roles in erythropoiesis. We found that the hematopoietic cell-specific bZip protein p45/NF-E2 interacts with T3 receptor (TR) and RA receptor (RAR) but not retinoid X receptor. The interaction is between the DNA-binding domain of the nuclear receptor and the leucine zipper region of p45/NF-E2 but is markedly enhanced by cognate ligand. Remarkably, ligand-dependent transactivation by TR and RAR is markedly potentiated by p45/NF-E2. This effect of p45/NF-E2 is prevented by maf-like protein p18, which functions positively as a heterodimer with p45/NF-E2 on DNA. Potentiation of hormone action by p45/NF-E2 requires its activation domain, which interacts strongly with the multifaceted coactivator cyclic AMP response element protein-binding protein (CBP). The region of CBP which interacts with p45/NF-E2 is the same interaction domain that mediates inhibition of hormone-stimulated transcription by AP1 transcription factors. Overexpression of the bZip interaction domain of CBP specifically abolishes the positive cross talk between TR and p45/NF-E2. Thus, positive cross talk between p45/NF-E2 and nuclear hormone receptors requires direct protein-protein interactions between these factors and with CBP, whose integration of positive signals from two transactivation domains provides a novel mechanism for potentiation of hormone action in hematopoietic cells.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

March 1997

Volume

17

Issue

3

Start / End Page

1407 / 1416

Location

United States

Related Subject Headings

  • Zinc Fingers
  • Viral Proteins
  • Triiodothyronine
  • Transcriptional Activation
  • Transcription Factors
  • Trans-Activators
  • Retinoid X Receptors
  • Retinoic Acid Receptor alpha
  • Recombinant Fusion Proteins
  • Receptors, Thyroid Hormone
 

Citation

APA
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ICMJE
MLA
NLM
Cheng, X., Reginato, M. J., Andrews, N. C., & Lazar, M. A. (1997). The transcriptional integrator CREB-binding protein mediates positive cross talk between nuclear hormone receptors and the hematopoietic bZip protein p45/NF-E2. Mol Cell Biol, 17(3), 1407–1416. https://doi.org/10.1128/MCB.17.3.1407
Cheng, X., M. J. Reginato, N. C. Andrews, and M. A. Lazar. “The transcriptional integrator CREB-binding protein mediates positive cross talk between nuclear hormone receptors and the hematopoietic bZip protein p45/NF-E2.Mol Cell Biol 17, no. 3 (March 1997): 1407–16. https://doi.org/10.1128/MCB.17.3.1407.
Cheng, X., et al. “The transcriptional integrator CREB-binding protein mediates positive cross talk between nuclear hormone receptors and the hematopoietic bZip protein p45/NF-E2.Mol Cell Biol, vol. 17, no. 3, Mar. 1997, pp. 1407–16. Pubmed, doi:10.1128/MCB.17.3.1407.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

March 1997

Volume

17

Issue

3

Start / End Page

1407 / 1416

Location

United States

Related Subject Headings

  • Zinc Fingers
  • Viral Proteins
  • Triiodothyronine
  • Transcriptional Activation
  • Transcription Factors
  • Trans-Activators
  • Retinoid X Receptors
  • Retinoic Acid Receptor alpha
  • Recombinant Fusion Proteins
  • Receptors, Thyroid Hormone