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Erythroid AP-1/NF-E2 elements vary in their response to NF-E2.

Publication ,  Journal Article
Walters, M; Andrews, NC; Magis, W; Martin, DI
Published in: Exp Hematol
February 1996

AP-1/NF-E2 motifs found in erythroid transcription control elements are associated with powerful transcription activation and thought to be regulated by the erythroid transcription factor NF-E2. We have studied AP-1/NF-E2 motifs from three different erythroid control elements (5'HS2 of the human beta-globin locus control region [LCR], the porphobilinogen deaminase [PBGD] promoter, and the mouse Band 3 promoter). We find that these AP-1/NF-E2 elements differ both in their ability to bind NF-E2 and their activity in transient assays. Each of the elements is bound by AP-1, but only the 5'HS2 and PBGD sites are bound by NF-E2. We examined the activity of these sites in minimal promoter constructs in transient assays. In erythroid cells, activity of duplicated NF-E2 motifs is positively correlated with binding by NF-E2; however, the Band 3 element not bound by NF-E2 is also active in some contexts. In HeLa cells, all sites were active and duplicated sites were most active. In F9 mouse teratocarcinoma cells, which express neither NF-E2 nor AP-1, the elements' activity parallels that in erythroid cells. While these finding are consistent with other evidence that NF-E2 is an important regulator of erythroid transcription, they suggest that some sites that resemble NF-E2 elements are actually regulated by other factors; we speculate that other tissue-specific and/or generally expressed factors may act on these sites.

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

February 1996

Volume

24

Issue

3

Start / End Page

445 / 452

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • Transcription Factor AP-1
  • Regulatory Sequences, Nucleic Acid
  • Rabbits
  • Promoter Regions, Genetic
  • NF-E2 Transcription Factor, p45 Subunit
  • NF-E2 Transcription Factor
  • Molecular Sequence Data
  • Mice
 

Citation

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ICMJE
MLA
NLM
Walters, M., Andrews, N. C., Magis, W., & Martin, D. I. (1996). Erythroid AP-1/NF-E2 elements vary in their response to NF-E2. Exp Hematol, 24(3), 445–452.
Walters, M., N. C. Andrews, W. Magis, and D. I. Martin. “Erythroid AP-1/NF-E2 elements vary in their response to NF-E2.Exp Hematol 24, no. 3 (February 1996): 445–52.
Walters M, Andrews NC, Magis W, Martin DI. Erythroid AP-1/NF-E2 elements vary in their response to NF-E2. Exp Hematol. 1996 Feb;24(3):445–52.
Walters, M., et al. “Erythroid AP-1/NF-E2 elements vary in their response to NF-E2.Exp Hematol, vol. 24, no. 3, Feb. 1996, pp. 445–52.
Walters M, Andrews NC, Magis W, Martin DI. Erythroid AP-1/NF-E2 elements vary in their response to NF-E2. Exp Hematol. 1996 Feb;24(3):445–452.
Journal cover image

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

February 1996

Volume

24

Issue

3

Start / End Page

445 / 452

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • Transcription Factor AP-1
  • Regulatory Sequences, Nucleic Acid
  • Rabbits
  • Promoter Regions, Genetic
  • NF-E2 Transcription Factor, p45 Subunit
  • NF-E2 Transcription Factor
  • Molecular Sequence Data
  • Mice