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Nitrosylation of manganese(II) tetrakis(N-ethylpyridinium-2-yl)porphyrin: a simple and sensitive spectrophotometric assay for nitric oxide.

Publication ,  Journal Article
Spasojevic, I; Batinic-Haberle, I; Fridovich, I
Published in: Nitric Oxide
October 2000

Reaction between NO(*) and manganese tetrakis(N-ethylpyridinium-2-yl)porphyrin (Mn(III)TE-2-PyP(5+)) was investigated at 25 degrees C. At high excess of NO(*) (1.5 mM) the reaction with the oxidized, air-stable form Mn(III)TE-2-PyP(5+) (5 microM), proceeds very slowly (t(1/2) congruent with 60 min). The presence of excess ascorbate (1 mM) produces the reduced form, Mn(II)TE-2-PyP(4+), which reacts with NO(*) stoichiometrically and in the time of mixing (k congruent with 1 x 10(6) M(-1) s(-1)). The high rate of formation and the stability of the product, Mn(II)TE-2-PyP(NO)(4+) (¿Mn(NO)¿(6)), make the reaction outcompete the reaction of NO(*) with O(2). Our in vitro measurements show a linear absorbance response upon addition of NO to a PBS, pH 7.4, solution containing an excess of ascorbate over Mn(III)TE-2-PyP(5+). Thus, the observed interactions can be the basis of a convenient and sensitive spectrophotometric assay for NO(*). Also, it may have important implications for the in vivo behavior of Mn(III)TE-2-PyP(5+) which is currently exploited as a possible therapeutic agent for various oxygen-radical related disorders.

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Published In

Nitric Oxide

DOI

ISSN

1089-8603

Publication Date

October 2000

Volume

4

Issue

5

Start / End Page

526 / 533

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Sensitivity and Specificity
  • Oxygen
  • Nitroso Compounds
  • Nitric Oxide
  • Molecular Structure
  • Metalloporphyrins
  • Calibration
  • Biochemistry & Molecular Biology
  • Ascorbic Acid
 

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Spasojevic, I., Batinic-Haberle, I., & Fridovich, I. (2000). Nitrosylation of manganese(II) tetrakis(N-ethylpyridinium-2-yl)porphyrin: a simple and sensitive spectrophotometric assay for nitric oxide. Nitric Oxide, 4(5), 526–533. https://doi.org/10.1006/niox.2000.0303
Spasojevic, I., I. Batinic-Haberle, and I. Fridovich. “Nitrosylation of manganese(II) tetrakis(N-ethylpyridinium-2-yl)porphyrin: a simple and sensitive spectrophotometric assay for nitric oxide.Nitric Oxide 4, no. 5 (October 2000): 526–33. https://doi.org/10.1006/niox.2000.0303.
Spasojevic, I., et al. “Nitrosylation of manganese(II) tetrakis(N-ethylpyridinium-2-yl)porphyrin: a simple and sensitive spectrophotometric assay for nitric oxide.Nitric Oxide, vol. 4, no. 5, Oct. 2000, pp. 526–33. Pubmed, doi:10.1006/niox.2000.0303.
Journal cover image

Published In

Nitric Oxide

DOI

ISSN

1089-8603

Publication Date

October 2000

Volume

4

Issue

5

Start / End Page

526 / 533

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Sensitivity and Specificity
  • Oxygen
  • Nitroso Compounds
  • Nitric Oxide
  • Molecular Structure
  • Metalloporphyrins
  • Calibration
  • Biochemistry & Molecular Biology
  • Ascorbic Acid