Sequence Requirements of the 5-Enolpyruvylshikimate-3-phosphate Synthase 5[prime]-Upstream Region for Tissue-Specific Expression in Flowers and Seedlings.
Journal Article (Journal Article)
We have analyzed expression from deletion derivatives of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) 5[prime]-upstream region in transgenic petunia flowers and seedlings. In seedlings, expression was strongest in root cortex cells and in trichomes. High-level expression in petals and in seedling roots was conferred by large (>500 base-pair) stretches of sequence, but was lost when smaller fragments were analyzed individually. This apparent requirement for extensive sequence suggests that combinations of cis-elements that are widely separated control tissue-specific expression from the EPSPS promoter. We have also used the high-level, petal-specific expression of the EPSPS promoter to change petal color in two mutant petunia lines.
Full Text
Duke Authors
Cited Authors
- Benfey, PN; Takatsuji, H; Ren, L; Shah, DM; Chua, NH
Published Date
- September 1990
Published In
Volume / Issue
- 2 / 9
Start / End Page
- 849 - 856
PubMed ID
- 12354968
Pubmed Central ID
- PMC159935
Electronic International Standard Serial Number (EISSN)
- 1532-298X
International Standard Serial Number (ISSN)
- 1040-4651
Digital Object Identifier (DOI)
- 10.1105/tpc.2.9.849
Language
- eng