The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants.

Journal Article (Journal Article)

Appropriate regulation of transcription in higher plants requires specific cis elements in the regulatory regions of genes and their corresponding trans-acting proteins. Analysis of the cauliflower mosaic virus (CaMV) 35S promoter has contributed to the understanding of transcriptional regulatory mechanisms. The intact 35S promoter confers constitutive expression upon heterologous genes in most plants. Dissection into subdomains that are able to confer tissue-specific gene expression has demonstrated that the promoter has a modular organization. When selected subdomains are combined, they confer expression not detected from the isolated subdomains, suggesting that synergistic interactions occur among cis elements. The expression patterns conferred by specific combinations of 35S subdomains differ in tobacco and petunia. This indicates that a combinatorial code of cisregulatory elements may be interpreted differently in different species.

Full Text

Duke Authors

Cited Authors

  • Benfey, PN; Chua, NH

Published Date

  • November 1990

Published In

Volume / Issue

  • 250 / 4983

Start / End Page

  • 959 - 966

PubMed ID

  • 17746920

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.250.4983.959


  • eng