The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns.

Published

Journal Article

We have analyzed expression conferred by two domains from the cauliflower mosaic virus (CaMV) 35S promoter and found different patterns in seeds, seedlings and seven week old plants. Expression from domain A (-90 to +8) is strongest in the radicle of the embryo, the radicle pole of the endosperm and in root tissue of seedlings and mature plants. Expression from domain B (-343 to -90) is strongest in the cells adjacent the cotyledon of the endosperm, in the cotyledons of the embryo and seedings and in the leaves and stem of mature plants. When both domain A and domain B are present expression is detectable in most tissues at all stages of development. Thus analysis of a constitutive promoter in transgenic plants can be used to identify cis elements that confer tissue specific and developmentally regulated expression.

Full Text

Duke Authors

Cited Authors

  • Benfey, PN; Ren, L; Chua, NH

Published Date

  • August 1, 1989

Published In

Volume / Issue

  • 8 / 8

Start / End Page

  • 2195 - 2202

PubMed ID

  • 16453896

Pubmed Central ID

  • 16453896

Electronic International Standard Serial Number (EISSN)

  • 1460-2075

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1002/j.1460-2075.1989.tb08342.x

Language

  • eng