The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines
Objective: Because HER-2/neu is overexpressed in one third of breast and ovarian cancers, we examined the effect of unconjugated monoclonal antibodies (ID-5, PB-3, TA-1) and an immunotoxin (TA-1-ricin) reactive with this protooncogene on the growth of breast and ovarian cancer cell lines. Study Design: The tritiated thymidine incorporation assay was used to examine the effect of unconjugated antibodies on proliferation. A limiting dilution clonogenic assay was used to assess the effect of immunotoxin on cellular cytotoxicity. Results: Scatchard analysis revealed that OVCA 420, OVCA 429, OVCA 432, and OVCA 433 cells had approximately 104 HER-2/neu receptors per cell, whereas the SKOv3 and SKBr3 cell lines expressed 105 and 106 receptors per cell, respectively. Monoclonal antibody ID5 caused significant inhibition of tritiated thymidine incorporation in SKBr3, SKOv3, and OVCA 420 cells (p < 0.002). The TA-1-ricin immunotoxin significantly inhibited the clonogenic growth of only SKBr3 and SKOv3 cells. Conclusion: HER-2/neu may be a useful target for immunotherapy with unconjugated antibodies and immunotoxins in ovarian and breast cancers that overexpress this protooncogene. © 1993, Mosby. All rights reserved.
Rodriguez, GC; Boente, MP; Berchuck, A; Whitaker, RS; O’Briant, KC; Xu, F; Bast, RC
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