Crystallization of a complex of cro repressor with a 17 base-pair operator.
Publication
, Journal Article
Brennan, RG; Takeda, Y; Kim, J; Anderson, WF; Matthews, BW
Published in: J Mol Biol
March 5, 1986
Crystals of the lambda cro repressor complexed to a 17 base-pair synthetic binding site related to the OR3 operator have been obtained. The complex crystallizes in the hexagonal space group P6(2) (or P6(4)) with unit cell dimensions a = b = 154.8 A, c = 85.6 A. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 A. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.
Duke Scholars
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
March 5, 1986
Volume
188
Issue
1
Start / End Page
115 / 118
Location
Netherlands
Related Subject Headings
- X-Ray Diffraction
- Viral Regulatory and Accessory Proteins
- Viral Proteins
- Transcription Factors
- Repressor Proteins
- Operator Regions, Genetic
- Macromolecular Substances
- DNA-Binding Proteins
- Crystallization
- Biochemistry & Molecular Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Brennan, R. G., Takeda, Y., Kim, J., Anderson, W. F., & Matthews, B. W. (1986). Crystallization of a complex of cro repressor with a 17 base-pair operator. J Mol Biol, 188(1), 115–118. https://doi.org/10.1016/0022-2836(86)90488-2
Brennan, R. G., Y. Takeda, J. Kim, W. F. Anderson, and B. W. Matthews. “Crystallization of a complex of cro repressor with a 17 base-pair operator.” J Mol Biol 188, no. 1 (March 5, 1986): 115–18. https://doi.org/10.1016/0022-2836(86)90488-2.
Brennan RG, Takeda Y, Kim J, Anderson WF, Matthews BW. Crystallization of a complex of cro repressor with a 17 base-pair operator. J Mol Biol. 1986 Mar 5;188(1):115–8.
Brennan, R. G., et al. “Crystallization of a complex of cro repressor with a 17 base-pair operator.” J Mol Biol, vol. 188, no. 1, Mar. 1986, pp. 115–18. Pubmed, doi:10.1016/0022-2836(86)90488-2.
Brennan RG, Takeda Y, Kim J, Anderson WF, Matthews BW. Crystallization of a complex of cro repressor with a 17 base-pair operator. J Mol Biol. 1986 Mar 5;188(1):115–118.
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
March 5, 1986
Volume
188
Issue
1
Start / End Page
115 / 118
Location
Netherlands
Related Subject Headings
- X-Ray Diffraction
- Viral Regulatory and Accessory Proteins
- Viral Proteins
- Transcription Factors
- Repressor Proteins
- Operator Regions, Genetic
- Macromolecular Substances
- DNA-Binding Proteins
- Crystallization
- Biochemistry & Molecular Biology