Crystallization of a complex of cro repressor with a 17 base-pair operator.

Published

Journal Article

Crystals of the lambda cro repressor complexed to a 17 base-pair synthetic binding site related to the OR3 operator have been obtained. The complex crystallizes in the hexagonal space group P6(2) (or P6(4)) with unit cell dimensions a = b = 154.8 A, c = 85.6 A. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 A. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.

Full Text

Duke Authors

Cited Authors

  • Brennan, RG; Takeda, Y; Kim, J; Anderson, WF; Matthews, BW

Published Date

  • March 5, 1986

Published In

Volume / Issue

  • 188 / 1

Start / End Page

  • 115 - 118

PubMed ID

  • 2940371

Pubmed Central ID

  • 2940371

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1016/0022-2836(86)90488-2

Language

  • eng

Conference Location

  • England