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Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG).

Publication ,  Journal Article
Brennan, RG; Sundaralingam, M
Published in: J Mol Biol
February 20, 1985

Crystals of the self-complementary decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG) have been grown from a solution containing [Co(NH3)6]Cl3 and spermine. The amber-colored crystals are hexagonal and belong to the space group P6(5) (or P6(1] with unit cell parameters a = 17.93 A, c = 43.41 A. Precession photography and molecular packing considerations indicate that the unit cell consists of a 12 nucleotide duplex. The asymmetric unit, therefore, is a disordered duplex dimer in which each pyrimidine-purine base-pair is occupied 60% of the time by a C . G pair and 40% of the time by a T . A pair. The above considerations and preliminary structure analysis reveal that this alternating pyrimidine-purine oligomer assumes a Z-DNA conformation.

Duke Scholars

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

February 20, 1985

Volume

181

Issue

4

Start / End Page

561 / 563

Location

Netherlands

Related Subject Headings

  • Oligonucleotides
  • Oligodeoxyribonucleotides
  • Crystallography
  • Biochemistry & Molecular Biology
  • Base Sequence
  • 3107 Microbiology
  • 3101 Biochemistry and cell biology
  • 0605 Microbiology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

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Brennan, R. G., & Sundaralingam, M. (1985). Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG). J Mol Biol, 181(4), 561–563. https://doi.org/10.1016/0022-2836(85)90429-2
Brennan, R. G., and M. Sundaralingam. “Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG).J Mol Biol 181, no. 4 (February 20, 1985): 561–63. https://doi.org/10.1016/0022-2836(85)90429-2.
Brennan, R. G., and M. Sundaralingam. “Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG).J Mol Biol, vol. 181, no. 4, Feb. 1985, pp. 561–63. Pubmed, doi:10.1016/0022-2836(85)90429-2.
Journal cover image

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

February 20, 1985

Volume

181

Issue

4

Start / End Page

561 / 563

Location

Netherlands

Related Subject Headings

  • Oligonucleotides
  • Oligodeoxyribonucleotides
  • Crystallography
  • Biochemistry & Molecular Biology
  • Base Sequence
  • 3107 Microbiology
  • 3101 Biochemistry and cell biology
  • 0605 Microbiology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry