Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG).
Publication
, Journal Article
Brennan, RG; Sundaralingam, M
Published in: J Mol Biol
February 20, 1985
Crystals of the self-complementary decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG) have been grown from a solution containing [Co(NH3)6]Cl3 and spermine. The amber-colored crystals are hexagonal and belong to the space group P6(5) (or P6(1] with unit cell parameters a = 17.93 A, c = 43.41 A. Precession photography and molecular packing considerations indicate that the unit cell consists of a 12 nucleotide duplex. The asymmetric unit, therefore, is a disordered duplex dimer in which each pyrimidine-purine base-pair is occupied 60% of the time by a C . G pair and 40% of the time by a T . A pair. The above considerations and preliminary structure analysis reveal that this alternating pyrimidine-purine oligomer assumes a Z-DNA conformation.
Duke Scholars
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
February 20, 1985
Volume
181
Issue
4
Start / End Page
561 / 563
Location
Netherlands
Related Subject Headings
- Oligonucleotides
- Oligodeoxyribonucleotides
- Crystallography
- Biochemistry & Molecular Biology
- Base Sequence
- 3107 Microbiology
- 3101 Biochemistry and cell biology
- 0605 Microbiology
- 0601 Biochemistry and Cell Biology
- 0304 Medicinal and Biomolecular Chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Brennan, R. G., & Sundaralingam, M. (1985). Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG). J Mol Biol, 181(4), 561–563. https://doi.org/10.1016/0022-2836(85)90429-2
Brennan, R. G., and M. Sundaralingam. “Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG).” J Mol Biol 181, no. 4 (February 20, 1985): 561–63. https://doi.org/10.1016/0022-2836(85)90429-2.
Brennan RG, Sundaralingam M. Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG). J Mol Biol. 1985 Feb 20;181(4):561–3.
Brennan, R. G., and M. Sundaralingam. “Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG).” J Mol Biol, vol. 181, no. 4, Feb. 1985, pp. 561–63. Pubmed, doi:10.1016/0022-2836(85)90429-2.
Brennan RG, Sundaralingam M. Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG). J Mol Biol. 1985 Feb 20;181(4):561–563.
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
February 20, 1985
Volume
181
Issue
4
Start / End Page
561 / 563
Location
Netherlands
Related Subject Headings
- Oligonucleotides
- Oligodeoxyribonucleotides
- Crystallography
- Biochemistry & Molecular Biology
- Base Sequence
- 3107 Microbiology
- 3101 Biochemistry and cell biology
- 0605 Microbiology
- 0601 Biochemistry and Cell Biology
- 0304 Medicinal and Biomolecular Chemistry