Endogenous polymers of ADP-ribose are associated with the nuclear matrix.

Published

Journal Article

The metabolism of nuclear polymers of ADP-ribose has been implicated in several chromatin-associated processes. However, the distribution of endogenous ADP-ribose polymers in the nucleus or within different fractions of chromatin has not been studied. Using a procedure which allowed the radiolabeling and detection of endogenous polymers of ADP-ribose, we have analyzed the nuclear distribution of these polymers in untreated cells and in cells subjected to hyperthermia, N-methyl-N'-nitro-N-nitrosoguanidine, or both. When isolated nuclei from cells subjected to any of these conditions were digested with micrococcal nuclease such that 80% of the DNA was released, 90% of the total poly(ADP-ribose) remained with the micrococcal nuclease resistant chromatin fraction. When nuclear matrix fractions were prepared by exhaustive DNase I digestion in combination with three different salt extraction procedures (2 M NaCl, 300 mM (NH4)2SO4 or 25 mM lithium diiodosalicylate), the matrices contained less than 1% of the total nuclear DNA but 50 to 70% of the total poly(ADP-ribose). These data suggest that the nuclear matrix may be a major site of poly(ADP-ribose) metabolism.

Full Text

Duke Authors

Cited Authors

  • Cardenas-Corona, ME; Jacobson, EL; Jacobson, MK

Published Date

  • November 5, 1987

Published In

Volume / Issue

  • 262 / 31

Start / End Page

  • 14863 - 14866

PubMed ID

  • 3667608

Pubmed Central ID

  • 3667608

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States