Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development.

Journal Article (Journal Article)

BACKGROUND/AIMS: Recent evidence suggests that CCAAT/enhancer binding protein (C/EBP) transcription factors may regulate hepatocyte terminal differentiation. METHODS: To explore this possibility, the present study looked for variations in the expression or DNA binding activity of different C/EBP isoforms during rat postnatal liver development and determined which of the C/EBPs were expressed by adult hepatocytes in primary culture. RESULTS: In intact rats, hepatocyte proliferation is active for 2-3 weeks after birth. During this period of postnatal liver growth, several liver-specific functions emerge and C/EBP alpha, beta, and delta isoforms are induced. Nuclear expression of the 36-kilodalton C/EBP delta protein increases immediately after birth, followed first by increases in the 38-kilodalton C/EBP beta protein expression and then by increases in the 42-kilodalton C/EBP alpha protein expression. Changes in C/EBP DNA binding activity accompany developmental increases in C/EBP proteins. Messenger RNAs of all three C/EBP isoforms are expressed by mature hepatocytes in primary culture. CONCLUSIONS: Specific C/EBP isoforms are induced differentially during the course of rat postnatal liver development. Young adult rats and cultured adult hepatocytes express all three C/EBP isoforms. These results are consistent with (but do not prove) the theory that variations in C/EBP expression and function help regulate hepatocyte terminal differentiation.

Full Text

Duke Authors

Cited Authors

  • Diehl, AM; Michaelson, P; Yang, SQ

Published Date

  • June 1994

Published In

Volume / Issue

  • 106 / 6

Start / End Page

  • 1625 - 1637

PubMed ID

  • 7910803

International Standard Serial Number (ISSN)

  • 0016-5085

Digital Object Identifier (DOI)

  • 10.1016/0016-5085(94)90420-0


  • eng

Conference Location

  • United States