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Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure.

Publication ,  Journal Article
Ding, B; Price, RL; Goldsmith, EC; Borg, TK; Yan, X; Douglas, PS; Weinberg, EO; Bartunek, J; Thielen, T; Didenko, VV; Lorell, BH
Published in: Circulation
June 20, 2000

BACKGROUND: To determine potential mechanisms of the transition from hypertrophy to very early failure, we examined apoptosis in a model of ascending aortic stenosis (AS) in male FVB/n mice. METHODS AND RESULTS: Compared with age-matched controls, 4-week and 7-week AS animals (n=12 to 16 per group) had increased ratios of left ventricular weight to body weight (4.7+/-0.7 versus 3.1+/-0.2 and 5. 7+/-0.4 versus 2.7+/-0.1 mg/g, respectively, P<0.05) with similar body weights. Myocyte width was also increased in 4-week and 7-week AS mice compared with controls (19.0+/-0.8 and 25.2+/-1.8 versus 14. 1+/-0.5 microm, respectively, P<0.01). By 7 weeks, AS myocytes displayed branching with distinct differences in intercalated disk size and staining for beta(1)-integrin on both cell surface and adjacent extracellular matrix. In vivo left ventricular systolic developed pressure per gram as well as endocardial fractional shortening were similar in 4-week AS and controls but depressed in 7-week AS mice. Myocyte apoptosis estimated by in situ nick end-labeling (TUNEL) was extremely rare in 4-week AS and control mice; however, a low prevalence of TUNEL-positive myocytes and DNA laddering were detected in 7-week AS mice. The specificity of TUNEL labeling was confirmed by in situ ligation of hairpin oligonucleotides. CONCLUSIONS: Our findings indicate that myocyte apoptosis develops during the transition from hypertrophy to early failure in mice with chronic biomechanical stress and support the hypothesis that the disruption of normal myocyte anchorage to adjacent extracellular matrix and cells, a process called anoikis, may signal apoptosis.

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Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

June 20, 2000

Volume

101

Issue

24

Start / End Page

2854 / 2862

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Microscopy, Confocal
  • Mice, Inbred Strains
  • Mice
  • Male
  • Integrin beta1
  • Hypertrophy, Left Ventricular
  • Hemodynamics
  • Echocardiography
  • Disease Progression
 

Citation

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Ding, B., Price, R. L., Goldsmith, E. C., Borg, T. K., Yan, X., Douglas, P. S., … Lorell, B. H. (2000). Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure. Circulation, 101(24), 2854–2862. https://doi.org/10.1161/01.cir.101.24.2854
Ding, B., R. L. Price, E. C. Goldsmith, T. K. Borg, X. Yan, P. S. Douglas, E. O. Weinberg, et al. “Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure.Circulation 101, no. 24 (June 20, 2000): 2854–62. https://doi.org/10.1161/01.cir.101.24.2854.
Ding B, Price RL, Goldsmith EC, Borg TK, Yan X, Douglas PS, et al. Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure. Circulation. 2000 Jun 20;101(24):2854–62.
Ding, B., et al. “Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure.Circulation, vol. 101, no. 24, June 2000, pp. 2854–62. Pubmed, doi:10.1161/01.cir.101.24.2854.
Ding B, Price RL, Goldsmith EC, Borg TK, Yan X, Douglas PS, Weinberg EO, Bartunek J, Thielen T, Didenko VV, Lorell BH. Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure. Circulation. 2000 Jun 20;101(24):2854–2862.

Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

June 20, 2000

Volume

101

Issue

24

Start / End Page

2854 / 2862

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Microscopy, Confocal
  • Mice, Inbred Strains
  • Mice
  • Male
  • Integrin beta1
  • Hypertrophy, Left Ventricular
  • Hemodynamics
  • Echocardiography
  • Disease Progression