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The conserved phosphoinositide 3-kinase pathway determines heart size in mice.

Publication ,  Journal Article
Shioi, T; Kang, PM; Douglas, PS; Hampe, J; Yballe, CM; Lawitts, J; Cantley, LC; Izumo, S
Published in: EMBO J
June 1, 2000

Phosphoinositide 3-kinase (PI3K) has been shown to regulate cell and organ size in Drosophila, but the role of PI3K in vertebrates in vivo is not well understood. To examine the role of PI3K in intact mammalian tissue, we have created and characterized transgenic mice expressing constitutively active or dominant-negative mutants of PI3K in the heart. Cardiac- specific expression of constitutively active PI3K resulted in mice with larger hearts, while dominant-negative PI3K resulted in mice with smaller hearts. The increase or decrease in heart size was associated with comparable increase or decrease in myocyte size. Cardiomyopathic changes, such as myocyte necrosis, apoptosis, interstitial fibrosis or contractile dysfunction, were not observed in either of the transgenic mice. Thus, the PI3K pathway is necessary and sufficient to promote organ growth in mammals.

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

June 1, 2000

Volume

19

Issue

11

Start / End Page

2537 / 2548

Location

England

Related Subject Headings

  • Ventricular Function, Left
  • Signal Transduction
  • Ribosomal Protein S6 Kinases
  • Recombinant Fusion Proteins
  • Protein Processing, Post-Translational
  • Phosphorylation
  • Phosphatidylinositol 3-Kinases
  • Organ Size
  • Myosin Heavy Chains
  • Myocardium
 

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Shioi, T., Kang, P. M., Douglas, P. S., Hampe, J., Yballe, C. M., Lawitts, J., … Izumo, S. (2000). The conserved phosphoinositide 3-kinase pathway determines heart size in mice. EMBO J, 19(11), 2537–2548. https://doi.org/10.1093/emboj/19.11.2537
Shioi, T., P. M. Kang, P. S. Douglas, J. Hampe, C. M. Yballe, J. Lawitts, L. C. Cantley, and S. Izumo. “The conserved phosphoinositide 3-kinase pathway determines heart size in mice.EMBO J 19, no. 11 (June 1, 2000): 2537–48. https://doi.org/10.1093/emboj/19.11.2537.
Shioi T, Kang PM, Douglas PS, Hampe J, Yballe CM, Lawitts J, et al. The conserved phosphoinositide 3-kinase pathway determines heart size in mice. EMBO J. 2000 Jun 1;19(11):2537–48.
Shioi, T., et al. “The conserved phosphoinositide 3-kinase pathway determines heart size in mice.EMBO J, vol. 19, no. 11, June 2000, pp. 2537–48. Pubmed, doi:10.1093/emboj/19.11.2537.
Shioi T, Kang PM, Douglas PS, Hampe J, Yballe CM, Lawitts J, Cantley LC, Izumo S. The conserved phosphoinositide 3-kinase pathway determines heart size in mice. EMBO J. 2000 Jun 1;19(11):2537–2548.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

June 1, 2000

Volume

19

Issue

11

Start / End Page

2537 / 2548

Location

England

Related Subject Headings

  • Ventricular Function, Left
  • Signal Transduction
  • Ribosomal Protein S6 Kinases
  • Recombinant Fusion Proteins
  • Protein Processing, Post-Translational
  • Phosphorylation
  • Phosphatidylinositol 3-Kinases
  • Organ Size
  • Myosin Heavy Chains
  • Myocardium