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In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation.

Publication ,  Journal Article
Manning, WJ; Wei, JY; Katz, SE; Litwin, SE; Douglas, PS
Published in: Am J Physiol
April 1994

Left ventricular (LV) mass is an important descriptor of cardiac status that increases with normal aging and may be affected by a variety of disease processes. There are currently limited noninvasive techniques that permit accurate determination of in vivo LV mass in very small animals, such as the mouse, a frequently used model for cardiac research. We sought to evaluate the ability of high-frequency (7.0 or 7.5 MHz), two-dimensional (2-D) guided M-mode echocardiography to estimate in vivo LV mass in the mouse. Fifteen adult mice weighing 22-45 g were studied, including six young adult (2- to 3-mo-old), two adult (12- to 14-mo-old), and seven senescent (18- to 20-mo-old) animals. Resting heart rate varied up to 450 beats/min. Anterior wall, inferior wall, and end-diastolic dimensions were measured, and echocardiographic LV mass (LVMe) was calculated using an uncorrected cube approximation. Autopsy LV mass was determined within 4 h of echocardiographic examination. Autopsy LV mass ranged from 88 to 211 mg. LV chamber dimensions included anterior wall (1.0 +/- 0.2 mm), inferior wall (1.1 +/- 0.3 mm), and end-diastolic dimension (3.7 +/- 0.5 mm). There was a very good correlation between LVMe (x) and autopsy LV mass (y):y = 0.96x - 7, r = 0.94, standard error of the estimate = 18 mg, P < 0.001. This correlation was stronger than that for autopsy LV mass and body weight (r = 0.70) or age (r = 0.74), indexes which until now were the only noninvasive correlates available for this very small animal model. We conclude that, despite the rapid heart rate and small size of the mouse heart, these results demonstrate the potential of high-frequency 2-D guided M-mode transthoracic echocardiography for the in vivo assessment of LV dimensions and mass in the mouse and may prove useful for cardiac research on aging and cardiomyopathies.

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Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

April 1994

Volume

266

Issue

4 Pt 2

Start / End Page

H1672 / H1675

Location

United States

Related Subject Headings

  • Organ Size
  • Myocardium
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Evaluation Studies as Topic
  • Echocardiography
  • Cardiovascular System & Hematology
  • Animals
  • Aging
 

Citation

APA
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ICMJE
MLA
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Manning, W. J., Wei, J. Y., Katz, S. E., Litwin, S. E., & Douglas, P. S. (1994). In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation. Am J Physiol, 266(4 Pt 2), H1672–H1675. https://doi.org/10.1152/ajpheart.1994.266.4.H1672
Manning, W. J., J. Y. Wei, S. E. Katz, S. E. Litwin, and P. S. Douglas. “In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation.Am J Physiol 266, no. 4 Pt 2 (April 1994): H1672–75. https://doi.org/10.1152/ajpheart.1994.266.4.H1672.
Manning WJ, Wei JY, Katz SE, Litwin SE, Douglas PS. In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation. Am J Physiol. 1994 Apr;266(4 Pt 2):H1672–5.
Manning, W. J., et al. “In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation.Am J Physiol, vol. 266, no. 4 Pt 2, Apr. 1994, pp. H1672–75. Pubmed, doi:10.1152/ajpheart.1994.266.4.H1672.
Manning WJ, Wei JY, Katz SE, Litwin SE, Douglas PS. In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation. Am J Physiol. 1994 Apr;266(4 Pt 2):H1672–H1675.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

April 1994

Volume

266

Issue

4 Pt 2

Start / End Page

H1672 / H1675

Location

United States

Related Subject Headings

  • Organ Size
  • Myocardium
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Evaluation Studies as Topic
  • Echocardiography
  • Cardiovascular System & Hematology
  • Animals
  • Aging