Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome.

Published

Journal Article

We report the existence of two structurally distinct forms of the angiotensin receptor AT-1 in the mouse. A Balb/c mouse genomic library was screened by homology screening with a polymerase chain reaction (PCR) amplified probe. Restriction mapping and sequencing of the isolated genes revealed the presence of two receptor isoforms, here named the mouse AT-1a and AT-1b receptors, containing 22 different amino acids. Receptor binding studies performed on COS-7 cells transfected with the two receptors revealed that they had similar binding profiles for angiotensin II, angiotensin III and AT-1 or AT-2 specific antagonists. Because many of the structural differences were in the carboxy terminal putative intracellular domain, we speculate that these isoforms may differ in their regulation, signal transduction, or desensitization mechanisms.

Full Text

Duke Authors

Cited Authors

  • Sasamura, H; Hein, L; Krieger, JE; Pratt, RE; Kobilka, BK; Dzau, VJ

Published Date

  • May 29, 1992

Published In

Volume / Issue

  • 185 / 1

Start / End Page

  • 253 - 259

PubMed ID

  • 1599461

Pubmed Central ID

  • 1599461

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/s0006-291x(05)80983-0

Language

  • eng

Conference Location

  • United States