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Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome.

Publication ,  Journal Article
Sasamura, H; Hein, L; Krieger, JE; Pratt, RE; Kobilka, BK; Dzau, VJ
Published in: Biochem Biophys Res Commun
May 29, 1992

We report the existence of two structurally distinct forms of the angiotensin receptor AT-1 in the mouse. A Balb/c mouse genomic library was screened by homology screening with a polymerase chain reaction (PCR) amplified probe. Restriction mapping and sequencing of the isolated genes revealed the presence of two receptor isoforms, here named the mouse AT-1a and AT-1b receptors, containing 22 different amino acids. Receptor binding studies performed on COS-7 cells transfected with the two receptors revealed that they had similar binding profiles for angiotensin II, angiotensin III and AT-1 or AT-2 specific antagonists. Because many of the structural differences were in the carboxy terminal putative intracellular domain, we speculate that these isoforms may differ in their regulation, signal transduction, or desensitization mechanisms.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 29, 1992

Volume

185

Issue

1

Start / End Page

253 / 259

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Receptors, Angiotensin
  • Multigene Family
  • Molecular Sequence Data
  • Mice
  • Genetic Variation
  • Cloning, Molecular
  • Biochemistry & Molecular Biology
  • Base Sequence
  • Animals
 

Citation

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Sasamura, H., Hein, L., Krieger, J. E., Pratt, R. E., Kobilka, B. K., & Dzau, V. J. (1992). Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome. Biochem Biophys Res Commun, 185(1), 253–259. https://doi.org/10.1016/s0006-291x(05)80983-0
Sasamura, H., L. Hein, J. E. Krieger, R. E. Pratt, B. K. Kobilka, and V. J. Dzau. “Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome.Biochem Biophys Res Commun 185, no. 1 (May 29, 1992): 253–59. https://doi.org/10.1016/s0006-291x(05)80983-0.
Sasamura H, Hein L, Krieger JE, Pratt RE, Kobilka BK, Dzau VJ. Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome. Biochem Biophys Res Commun. 1992 May 29;185(1):253–9.
Sasamura, H., et al. “Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome.Biochem Biophys Res Commun, vol. 185, no. 1, May 1992, pp. 253–59. Pubmed, doi:10.1016/s0006-291x(05)80983-0.
Sasamura H, Hein L, Krieger JE, Pratt RE, Kobilka BK, Dzau VJ. Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome. Biochem Biophys Res Commun. 1992 May 29;185(1):253–259.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 29, 1992

Volume

185

Issue

1

Start / End Page

253 / 259

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Receptors, Angiotensin
  • Multigene Family
  • Molecular Sequence Data
  • Mice
  • Genetic Variation
  • Cloning, Molecular
  • Biochemistry & Molecular Biology
  • Base Sequence
  • Animals