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Identification of negative and positive regulatory elements in the human renin gene.

Publication ,  Journal Article
Burt, DW; Nakamura, N; Kelley, P; Dzau, VJ
Published in: J Biol Chem
May 5, 1989

Renin gene expression is tissue-specific and under complex hormonal control. To investigate which DNA elements are involved in the control of human renin gene expression, we performed transient DNA transfer experiments with renin-chloramphenicol acetyltransferase fusions. We have mapped a complex arrangement of positive and negative control sequences in the 5' flanking region of the human renin gene. One positive control element is active in either orientation and defines a renin gene enhancer. The negative element is also active in either orientation and defines a renin gene silencer. Mapping in the same region as the silencer is a cAMP-responsive element, a sequence conserved in mouse, rat, and human renin genes.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 5, 1989

Volume

264

Issue

13

Start / End Page

7357 / 7362

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Sequence Homology, Nucleic Acid
  • Renin
  • Regulatory Sequences, Nucleic Acid
  • Rats
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Mice
  • Humans
 

Citation

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Burt, D. W., Nakamura, N., Kelley, P., & Dzau, V. J. (1989). Identification of negative and positive regulatory elements in the human renin gene. J Biol Chem, 264(13), 7357–7362.
Burt, D. W., N. Nakamura, P. Kelley, and V. J. Dzau. “Identification of negative and positive regulatory elements in the human renin gene.J Biol Chem 264, no. 13 (May 5, 1989): 7357–62.
Burt DW, Nakamura N, Kelley P, Dzau VJ. Identification of negative and positive regulatory elements in the human renin gene. J Biol Chem. 1989 May 5;264(13):7357–62.
Burt, D. W., et al. “Identification of negative and positive regulatory elements in the human renin gene.J Biol Chem, vol. 264, no. 13, May 1989, pp. 7357–62.
Burt DW, Nakamura N, Kelley P, Dzau VJ. Identification of negative and positive regulatory elements in the human renin gene. J Biol Chem. 1989 May 5;264(13):7357–7362.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 5, 1989

Volume

264

Issue

13

Start / End Page

7357 / 7362

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Sequence Homology, Nucleic Acid
  • Renin
  • Regulatory Sequences, Nucleic Acid
  • Rats
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Mice
  • Humans