Identification of negative and positive regulatory elements in the human renin gene.
Publication
, Journal Article
Burt, DW; Nakamura, N; Kelley, P; Dzau, VJ
Published in: J Biol Chem
May 5, 1989
Renin gene expression is tissue-specific and under complex hormonal control. To investigate which DNA elements are involved in the control of human renin gene expression, we performed transient DNA transfer experiments with renin-chloramphenicol acetyltransferase fusions. We have mapped a complex arrangement of positive and negative control sequences in the 5' flanking region of the human renin gene. One positive control element is active in either orientation and defines a renin gene enhancer. The negative element is also active in either orientation and defines a renin gene silencer. Mapping in the same region as the silencer is a cAMP-responsive element, a sequence conserved in mouse, rat, and human renin genes.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
May 5, 1989
Volume
264
Issue
13
Start / End Page
7357 / 7362
Location
United States
Related Subject Headings
- Tumor Cells, Cultured
- Transcription, Genetic
- Sequence Homology, Nucleic Acid
- Renin
- Regulatory Sequences, Nucleic Acid
- Rats
- Promoter Regions, Genetic
- Molecular Sequence Data
- Mice
- Humans
Citation
APA
Chicago
ICMJE
MLA
NLM
Burt, D. W., Nakamura, N., Kelley, P., & Dzau, V. J. (1989). Identification of negative and positive regulatory elements in the human renin gene. J Biol Chem, 264(13), 7357–7362.
Burt, D. W., N. Nakamura, P. Kelley, and V. J. Dzau. “Identification of negative and positive regulatory elements in the human renin gene.” J Biol Chem 264, no. 13 (May 5, 1989): 7357–62.
Burt DW, Nakamura N, Kelley P, Dzau VJ. Identification of negative and positive regulatory elements in the human renin gene. J Biol Chem. 1989 May 5;264(13):7357–62.
Burt, D. W., et al. “Identification of negative and positive regulatory elements in the human renin gene.” J Biol Chem, vol. 264, no. 13, May 1989, pp. 7357–62.
Burt DW, Nakamura N, Kelley P, Dzau VJ. Identification of negative and positive regulatory elements in the human renin gene. J Biol Chem. 1989 May 5;264(13):7357–7362.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
May 5, 1989
Volume
264
Issue
13
Start / End Page
7357 / 7362
Location
United States
Related Subject Headings
- Tumor Cells, Cultured
- Transcription, Genetic
- Sequence Homology, Nucleic Acid
- Renin
- Regulatory Sequences, Nucleic Acid
- Rats
- Promoter Regions, Genetic
- Molecular Sequence Data
- Mice
- Humans