Identification of negative and positive regulatory elements in the human renin gene.
Journal Article (Journal Article)
Renin gene expression is tissue-specific and under complex hormonal control. To investigate which DNA elements are involved in the control of human renin gene expression, we performed transient DNA transfer experiments with renin-chloramphenicol acetyltransferase fusions. We have mapped a complex arrangement of positive and negative control sequences in the 5' flanking region of the human renin gene. One positive control element is active in either orientation and defines a renin gene enhancer. The negative element is also active in either orientation and defines a renin gene silencer. Mapping in the same region as the silencer is a cAMP-responsive element, a sequence conserved in mouse, rat, and human renin genes.
Full Text
Duke Authors
Cited Authors
- Burt, DW; Nakamura, N; Kelley, P; Dzau, VJ
Published Date
- May 5, 1989
Published In
Volume / Issue
- 264 / 13
Start / End Page
- 7357 - 7362
PubMed ID
- 2540188
International Standard Serial Number (ISSN)
- 0021-9258
Language
- eng
Conference Location
- United States