Identification of negative and positive regulatory elements in the human renin gene.

Published

Journal Article

Renin gene expression is tissue-specific and under complex hormonal control. To investigate which DNA elements are involved in the control of human renin gene expression, we performed transient DNA transfer experiments with renin-chloramphenicol acetyltransferase fusions. We have mapped a complex arrangement of positive and negative control sequences in the 5' flanking region of the human renin gene. One positive control element is active in either orientation and defines a renin gene enhancer. The negative element is also active in either orientation and defines a renin gene silencer. Mapping in the same region as the silencer is a cAMP-responsive element, a sequence conserved in mouse, rat, and human renin genes.

Full Text

Duke Authors

Cited Authors

  • Burt, DW; Nakamura, N; Kelley, P; Dzau, VJ

Published Date

  • May 5, 1989

Published In

Volume / Issue

  • 264 / 13

Start / End Page

  • 7357 - 7362

PubMed ID

  • 2540188

Pubmed Central ID

  • 2540188

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States