Mapping of the human renin transcription start site: evidence for a single functional promoter.

Published

Journal Article

The 5' flanking region of the human renin gene contains two putative promoter sequences (TATA boxes), named P1 and P2. These are located in positions -77 to -71 and -29 to -23 respectively, each followed by a possible translational start site (AUG). In order to identify whether these sequences are functional in the kidney, we employed the RNAse protection assay. Total RNA extracted from human kidney cortex was hybridized with labeled cRNA probes complementary to the 5' flanking region of the human renin gene. After digestion with RNAse, protected hybrids were identified by electrophoresis on polyacrylamide/urea gels and autoradiography. Results showed that only P2 is active in human kidney resulting in a single transcript. This suggests that human kidney renin is synthesized as a single preproform.

Full Text

Duke Authors

Cited Authors

  • Paul, M; Burt, DW; Nakamura, N; Pratt, RE; Dzau, VJ

Published Date

  • 1988

Published In

Volume / Issue

  • 10 / 6

Start / End Page

  • 1313 - 1316

PubMed ID

  • 3066535

Pubmed Central ID

  • 3066535

International Standard Serial Number (ISSN)

  • 0730-0077

Digital Object Identifier (DOI)

  • 10.1080/07300077.1988.11878928

Language

  • eng

Conference Location

  • United States