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Functional human renin promoter in transfected cells: evidence for cell-specific expression.

Publication ,  Journal Article
Burt, DW; Nakamura, N; Kelley, P; Dzau, VJ
Published in: J Hypertens Suppl
December 1988

Expression of the human renin gene is regulated in a tissue-specific manner, but study of this regulation has been limited by a lack of suitable cell lines that simulate endogenous control. In order to characterize the regulation of renin gene expression, the 5' flanking region (892 base pairs) from the human renin gene was linked to the chloramphenicol acetyltransferase gene and was introduced into multiple human cell lines by calcium phosphate precipitation or electroporation methods to assess transcriptional control. The human renin promoter was active when transfected into cultured human choriocarcinoma cells (JEG-3) and rat vascular smooth muscle cells, but it was not active in many other cloned cell types. These results suggest that selective cell lines contain the specific trans-acting factors necessary for human renin gene expression, and support the concept of cell-specific expression of this gene.

Duke Scholars

Published In

J Hypertens Suppl

DOI

ISSN

0952-1178

Publication Date

December 1988

Volume

6

Issue

4

Start / End Page

S429 / S431

Location

England

Related Subject Headings

  • Transfection
  • Renin
  • Promoter Regions, Genetic
  • Humans
  • Gene Expression Regulation
  • Cell Line
  • Cell Differentiation
  • Cardiovascular System & Hematology
 

Citation

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Burt, D. W., Nakamura, N., Kelley, P., & Dzau, V. J. (1988). Functional human renin promoter in transfected cells: evidence for cell-specific expression. J Hypertens Suppl, 6(4), S429–S431. https://doi.org/10.1097/00004872-198812040-00135
Burt, D. W., N. Nakamura, P. Kelley, and V. J. Dzau. “Functional human renin promoter in transfected cells: evidence for cell-specific expression.J Hypertens Suppl 6, no. 4 (December 1988): S429–31. https://doi.org/10.1097/00004872-198812040-00135.
Burt DW, Nakamura N, Kelley P, Dzau VJ. Functional human renin promoter in transfected cells: evidence for cell-specific expression. J Hypertens Suppl. 1988 Dec;6(4):S429–31.
Burt, D. W., et al. “Functional human renin promoter in transfected cells: evidence for cell-specific expression.J Hypertens Suppl, vol. 6, no. 4, Dec. 1988, pp. S429–31. Pubmed, doi:10.1097/00004872-198812040-00135.
Burt DW, Nakamura N, Kelley P, Dzau VJ. Functional human renin promoter in transfected cells: evidence for cell-specific expression. J Hypertens Suppl. 1988 Dec;6(4):S429–S431.

Published In

J Hypertens Suppl

DOI

ISSN

0952-1178

Publication Date

December 1988

Volume

6

Issue

4

Start / End Page

S429 / S431

Location

England

Related Subject Headings

  • Transfection
  • Renin
  • Promoter Regions, Genetic
  • Humans
  • Gene Expression Regulation
  • Cell Line
  • Cell Differentiation
  • Cardiovascular System & Hematology