Different secretory pathways of renin from mouse cells transfected with the human renin gene.
Published
Journal Article
Mammalian cells in culture, transfected with human renin gene, can provide a useful tool for studying renin biosynthesis and secretion. We transfected fibroblast cells (mouse L929 and Chinese hamster ovary cells) and pituitary tumor cells (mouse AtT-20) with the human renin gene and a selectable plasmid (pSV2Neo). Transfected fibroblasts synthesize prorenin only. Prorenin is secreted by fibroblasts constitutively and the secretion is not influenced by 8-bromo-cAMP. On the other hand, transfected AtT-20 cells synthesized both prorenin and mature active renin. Transfected AtT-20 cells release prorenin by constitutive secretion but mature renin is secreted by a regulated mechanism since the secretion of the former is not influenced by 8-bromo-cAMP but the release of the latter is significantly stimulated. Our studies demonstrate that human renin may be secreted by at least two cellular pathways: prorenin by a constitutive pathway and mature renin by a regulated pathway. These transfected cells may provide useful models for studies of human renin synthesis, processing, and secretion.
Full Text
Duke Authors
Cited Authors
- Pratt, RE; Flynn, JA; Hobart, PM; Paul, M; Dzau, VJ
Published Date
- March 5, 1988
Published In
Volume / Issue
- 263 / 7
Start / End Page
- 3137 - 3141
PubMed ID
- 2893797
Pubmed Central ID
- 2893797
International Standard Serial Number (ISSN)
- 0021-9258
Language
- eng
Conference Location
- United States