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Different secretory pathways of renin from mouse cells transfected with the human renin gene.

Publication ,  Journal Article
Pratt, RE; Flynn, JA; Hobart, PM; Paul, M; Dzau, VJ
Published in: J Biol Chem
March 5, 1988

Mammalian cells in culture, transfected with human renin gene, can provide a useful tool for studying renin biosynthesis and secretion. We transfected fibroblast cells (mouse L929 and Chinese hamster ovary cells) and pituitary tumor cells (mouse AtT-20) with the human renin gene and a selectable plasmid (pSV2Neo). Transfected fibroblasts synthesize prorenin only. Prorenin is secreted by fibroblasts constitutively and the secretion is not influenced by 8-bromo-cAMP. On the other hand, transfected AtT-20 cells synthesized both prorenin and mature active renin. Transfected AtT-20 cells release prorenin by constitutive secretion but mature renin is secreted by a regulated mechanism since the secretion of the former is not influenced by 8-bromo-cAMP but the release of the latter is significantly stimulated. Our studies demonstrate that human renin may be secreted by at least two cellular pathways: prorenin by a constitutive pathway and mature renin by a regulated pathway. These transfected cells may provide useful models for studies of human renin synthesis, processing, and secretion.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

March 5, 1988

Volume

263

Issue

7

Start / End Page

3137 / 3141

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Renin
  • RNA, Messenger
  • Poly A
  • Plasmids
  • Pituitary Neoplasms
  • Ovary
  • Nucleic Acid Hybridization
  • Mice
 

Citation

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Pratt, R. E., Flynn, J. A., Hobart, P. M., Paul, M., & Dzau, V. J. (1988). Different secretory pathways of renin from mouse cells transfected with the human renin gene. J Biol Chem, 263(7), 3137–3141.
Pratt, R. E., J. A. Flynn, P. M. Hobart, M. Paul, and V. J. Dzau. “Different secretory pathways of renin from mouse cells transfected with the human renin gene.J Biol Chem 263, no. 7 (March 5, 1988): 3137–41.
Pratt RE, Flynn JA, Hobart PM, Paul M, Dzau VJ. Different secretory pathways of renin from mouse cells transfected with the human renin gene. J Biol Chem. 1988 Mar 5;263(7):3137–41.
Pratt, R. E., et al. “Different secretory pathways of renin from mouse cells transfected with the human renin gene.J Biol Chem, vol. 263, no. 7, Mar. 1988, pp. 3137–41.
Pratt RE, Flynn JA, Hobart PM, Paul M, Dzau VJ. Different secretory pathways of renin from mouse cells transfected with the human renin gene. J Biol Chem. 1988 Mar 5;263(7):3137–3141.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

March 5, 1988

Volume

263

Issue

7

Start / End Page

3137 / 3141

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Renin
  • RNA, Messenger
  • Poly A
  • Plasmids
  • Pituitary Neoplasms
  • Ovary
  • Nucleic Acid Hybridization
  • Mice