Different secretory pathways of renin from mouse cells transfected with the human renin gene.

Journal Article (Journal Article)

Mammalian cells in culture, transfected with human renin gene, can provide a useful tool for studying renin biosynthesis and secretion. We transfected fibroblast cells (mouse L929 and Chinese hamster ovary cells) and pituitary tumor cells (mouse AtT-20) with the human renin gene and a selectable plasmid (pSV2Neo). Transfected fibroblasts synthesize prorenin only. Prorenin is secreted by fibroblasts constitutively and the secretion is not influenced by 8-bromo-cAMP. On the other hand, transfected AtT-20 cells synthesized both prorenin and mature active renin. Transfected AtT-20 cells release prorenin by constitutive secretion but mature renin is secreted by a regulated mechanism since the secretion of the former is not influenced by 8-bromo-cAMP but the release of the latter is significantly stimulated. Our studies demonstrate that human renin may be secreted by at least two cellular pathways: prorenin by a constitutive pathway and mature renin by a regulated pathway. These transfected cells may provide useful models for studies of human renin synthesis, processing, and secretion.

Full Text

Duke Authors

Cited Authors

  • Pratt, RE; Flynn, JA; Hobart, PM; Paul, M; Dzau, VJ

Published Date

  • March 5, 1988

Published In

Volume / Issue

  • 263 / 7

Start / End Page

  • 3137 - 3141

PubMed ID

  • 2893797

International Standard Serial Number (ISSN)

  • 0021-9258


  • eng

Conference Location

  • United States