Studies of the biosynthesis of renin with a cell-free translation system.

Journal Article

1. Poly(A)+ mRNA from mouse submaxillary gland encodes a polypeptide of molecular weight 48 000 (48K polypeptide) which is abundant in the male. 2. This polypeptide is selectively absent in the translation products of mRNA from a strain of genetically renin-deficient mice C57 BL/10J. 3. The 48K polypeptide binds and co-elutes in identical fashion with pure authentic renin on pepstatin affinity chromatography. 4. Immunoprecipitation of translation products of male glandular mRNA with renin-specific antibody yielded this 48K band upon analysis by SDS/polyacrylamide gel electrophoresis and fluorography. Pure renin of molecular weight 37 000 blocked the binding of this polypeptide to antirenin antibody. 5. Mouse submaxillary gland synthesizes a renin precursor. The renin mRNA is androgenically regulated.

Full Text

Duke Authors

Cited Authors

  • Dzau, VJ; Ouellette, A; Pratt, R

Published Date

  • December 1, 1981

Published In

Volume / Issue

  • 61 Suppl 7 /

Start / End Page

  • 241s - 243s

PubMed ID

  • 7032813

International Standard Serial Number (ISSN)

  • 0143-5221

Language

  • eng

Conference Location

  • England