Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma.

Journal Article (Journal Article)

PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology. METHODS: Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma. RESULTS: Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls. CONCLUSIONS: Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.

Full Text

Duke Authors

Cited Authors

  • Goldhagen, B; Proia, AD; Epstein, DL; Rao, PV

Published Date

  • October 2012

Published In

Volume / Issue

  • 21 / 8

Start / End Page

  • 530 - 538

PubMed ID

  • 22495072

Electronic International Standard Serial Number (EISSN)

  • 1536-481X

Digital Object Identifier (DOI)

  • 10.1097/IJG.0b013e318241b83c


  • eng

Conference Location

  • United States