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Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae.

Publication ,  Journal Article
Gottlin-Ninfa, E; Kaback, DB
Published in: Molecular and cellular biology
June 1986

Strains of the yeast Saccharomyces cerevisiae that are heterozygous for the mating-type locus (MATa/MAT alpha) undergo meiosis and spore formation when they are starved for nitrogen and are provided with a nonfermentable carbon source such as potassium acetate. Haploids and diploids homozygous for the mating-type locus (MAT alpha/MAT alpha or MATa/MATa) are asporogenous and undergo neither meiosis nor spore formation when incubated under the same conditions. A small number of genes produce transcripts that appear to be induced specifically in sporulating cells. These transcripts either are not found or are present at much lower levels both in vegetatively growing cells and in cells from asporogenous strains that have been incubated in sporulation medium. Several genes complementary to these MATa/MAT alpha-dependent sporulation-induced transcripts were isolated from a gene-size insert yeast-lambda recombinant DNA library, by differential-plaque filter hybridization. An attempt was made to determine the function of three of these genes by mutating them in the yeast genome with in vitro mutagenesis and one-step gene replacement techniques. One gene was extensively disrupted by both a 0.3-kilobase deletion and the insertion of two large DNA sequences at different sites within the gene. Surprisingly, this compound mutation did not appear to affect meiosis or the production of viable ascospores, indicating that this gene was dispensable for differentiation. The other two genes were disrupted by simple insertion mutations at a site where it was possible that they might still possess some gene activity. These mutations also did not appear to affect sporulation. These results suggest that not all sporulation-induced genes are essential for meiosis and the production of viable ascospores under the conditions examined.

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Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

June 1986

Volume

6

Issue

6

Start / End Page

2185 / 2197

Related Subject Headings

  • Transcription, Genetic
  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Mutation
  • Genes, Mating Type, Fungal
  • Genes, Fungal
  • Gene Expression Regulation
  • Developmental Biology
  • Chromosome Mapping
  • 42 Health sciences
 

Citation

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Gottlin-Ninfa, E., & Kaback, D. B. (1986). Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae. Molecular and Cellular Biology, 6(6), 2185–2197. https://doi.org/10.1128/mcb.6.6.2185-2197.1986
Gottlin-Ninfa, E., and D. B. Kaback. “Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae.Molecular and Cellular Biology 6, no. 6 (June 1986): 2185–97. https://doi.org/10.1128/mcb.6.6.2185-2197.1986.
Gottlin-Ninfa E, Kaback DB. Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae. Molecular and cellular biology. 1986 Jun;6(6):2185–97.
Gottlin-Ninfa, E., and D. B. Kaback. “Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae.Molecular and Cellular Biology, vol. 6, no. 6, June 1986, pp. 2185–97. Epmc, doi:10.1128/mcb.6.6.2185-2197.1986.
Gottlin-Ninfa E, Kaback DB. Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae. Molecular and cellular biology. 1986 Jun;6(6):2185–2197.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

June 1986

Volume

6

Issue

6

Start / End Page

2185 / 2197

Related Subject Headings

  • Transcription, Genetic
  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Mutation
  • Genes, Mating Type, Fungal
  • Genes, Fungal
  • Gene Expression Regulation
  • Developmental Biology
  • Chromosome Mapping
  • 42 Health sciences