Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.

Journal Article (Journal Article)

Mitogen-activated protein kinase (MAP kinase) activator was purified 2000-fold from skeletal muscle, and proteins which co-purified with the activator were analysed after SDS/PAGE by renaturation and partial sequencing. Activity for tyrosine and threonine phosphorylation of MAP kinase was present in two bands of approx. 48 and 46 kDa, which have sequence similarity to small GTP-binding protein p25 GDP dissociation inhibitor and protein kinases (PBS2, SPK1+, STE7, BYR1) respectively.

Full Text

Duke Authors

Cited Authors

  • Wu, J; Michel, H; Rossomando, A; Haystead, T; Shabanowitz, J; Hunt, DF; Sturgill, TW

Published Date

  • August 1, 1992

Published In

Volume / Issue

  • 285 ( Pt 3) / Pt 3

Start / End Page

  • 701 - 705

PubMed ID

  • 1379797

Pubmed Central ID

  • PMC1132850

International Standard Serial Number (ISSN)

  • 0264-6021

Digital Object Identifier (DOI)

  • 10.1042/bj2850701


  • eng

Conference Location

  • England