Scholars@Duke publication: Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.

Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.

Publication , Journal Article

Wu, J; Michel, H; Rossomando, A; Haystead, T; Shabanowitz, J; Hunt, DF; Sturgill, TW

Published in: Biochem J

August 1, 1992

Mitogen-activated protein kinase (MAP kinase) activator was purified 2000-fold from skeletal muscle, and proteins which co-purified with the activator were analysed after SDS/PAGE by renaturation and partial sequencing. Activity for tyrosine and threonine phosphorylation of MAP kinase was present in two bands of approx. 48 and 46 kDa, which have sequence similarity to small GTP-binding protein p25 GDP dissociation inhibitor and protein kinases (PBS2, SPK1+, STE7, BYR1) respectively.

Duke Scholars

Author Timothy Arthur James Haystead Pharmacology & Cancer Biology

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Published In

Biochem J

DOI

10.1042/bj2850701

ISSN

0264-6021

Publication Date

August 1, 1992

Volume

285 ( Pt 3)

Issue

Pt 3

Start / End Page

701 / 705

Location

England

Related Subject Headings

Tyrosine
Sequence Homology, Nucleic Acid
Rabbits
Protein Kinases
Protein Denaturation
Phosphotyrosine
Phosphothreonine
Phosphorylation
Muscles
Molecular Weight

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Wu, J., Michel, H., Rossomando, A., Haystead, T., Shabanowitz, J., Hunt, D. F., & Sturgill, T. W. (1992). Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle. Biochem J, 285 ( Pt 3)(Pt 3), 701–705. https://doi.org/10.1042/bj2850701

Wu, J., H. Michel, A. Rossomando, T. Haystead, J. Shabanowitz, D. F. Hunt, and T. W. Sturgill. “Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.” Biochem J 285 ( Pt 3), no. Pt 3 (August 1, 1992): 701–5. https://doi.org/10.1042/bj2850701.

Wu J, Michel H, Rossomando A, Haystead T, Shabanowitz J, Hunt DF, et al. Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle. Biochem J. 1992 Aug 1;285 ( Pt 3)(Pt 3):701–5.

Wu, J., et al. “Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.” Biochem J, vol. 285 ( Pt 3), no. Pt 3, Aug. 1992, pp. 701–05. Pubmed, doi:10.1042/bj2850701.

Wu J, Michel H, Rossomando A, Haystead T, Shabanowitz J, Hunt DF, Sturgill TW. Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle. Biochem J. 1992 Aug 1;285 ( Pt 3)(Pt 3):701–705.