Mouse liver cell culture. II. Primary culture.

Published

Journal Article

Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minimal attachment (less than 7%) occurred in serumless medium. Serum concentrations above 10% and attachment durations greater that 2 h resulted in no increased attachment of viable cells. Nonviable cells, however, progressively attached when both of these parameters were increased. Survival data of the cells in culture resembled those reported for rat hepatocytes in primary culture. A progressive decrease in survival was noted following initial attachment until only approximately 15% of initially plated cells remained viable and attached after 8 d culture. The decrease in survival was accompanied by morphologic changes including flattening and elongation of the cells, some multinucleation, and disruption of monolayer groups.

Full Text

Duke Authors

Cited Authors

  • Klaunig, JE; Goldblatt, PJ; Hinton, DE; Lipsky, MM; Trump, BF

Published Date

  • October 1, 1981

Published In

Volume / Issue

  • 17 / 10

Start / End Page

  • 926 - 934

PubMed ID

  • 7309042

Pubmed Central ID

  • 7309042

International Standard Serial Number (ISSN)

  • 0073-5655

Digital Object Identifier (DOI)

  • 10.1007/bf02618289

Language

  • eng