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Mouse liver cell culture. II. Primary culture.

Publication ,  Journal Article
Klaunig, JE; Goldblatt, PJ; Hinton, DE; Lipsky, MM; Trump, BF
Published in: In vitro
October 1981

Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minimal attachment (less than 7%) occurred in serumless medium. Serum concentrations above 10% and attachment durations greater that 2 h resulted in no increased attachment of viable cells. Nonviable cells, however, progressively attached when both of these parameters were increased. Survival data of the cells in culture resembled those reported for rat hepatocytes in primary culture. A progressive decrease in survival was noted following initial attachment until only approximately 15% of initially plated cells remained viable and attached after 8 d culture. The decrease in survival was accompanied by morphologic changes including flattening and elongation of the cells, some multinucleation, and disruption of monolayer groups.

Duke Scholars

Published In

In vitro

DOI

ISSN

0073-5655

Publication Date

October 1981

Volume

17

Issue

10

Start / End Page

926 / 934

Related Subject Headings

  • Mice, Inbred BALB C
  • Mice
  • Male
  • Liver
  • Cytoplasm
  • Culture Media
  • Cells, Cultured
  • Cell Survival
  • Cell Separation
  • Cell Nucleus
 

Citation

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Klaunig, J. E., Goldblatt, P. J., Hinton, D. E., Lipsky, M. M., & Trump, B. F. (1981). Mouse liver cell culture. II. Primary culture. In Vitro, 17(10), 926–934. https://doi.org/10.1007/bf02618289
Klaunig, J. E., P. J. Goldblatt, D. E. Hinton, M. M. Lipsky, and B. F. Trump. “Mouse liver cell culture. II. Primary culture.In Vitro 17, no. 10 (October 1981): 926–34. https://doi.org/10.1007/bf02618289.
Klaunig JE, Goldblatt PJ, Hinton DE, Lipsky MM, Trump BF. Mouse liver cell culture. II. Primary culture. In vitro. 1981 Oct;17(10):926–34.
Klaunig, J. E., et al. “Mouse liver cell culture. II. Primary culture.In Vitro, vol. 17, no. 10, Oct. 1981, pp. 926–34. Epmc, doi:10.1007/bf02618289.
Klaunig JE, Goldblatt PJ, Hinton DE, Lipsky MM, Trump BF. Mouse liver cell culture. II. Primary culture. In vitro. 1981 Oct;17(10):926–934.

Published In

In vitro

DOI

ISSN

0073-5655

Publication Date

October 1981

Volume

17

Issue

10

Start / End Page

926 / 934

Related Subject Headings

  • Mice, Inbred BALB C
  • Mice
  • Male
  • Liver
  • Cytoplasm
  • Culture Media
  • Cells, Cultured
  • Cell Survival
  • Cell Separation
  • Cell Nucleus