α2 -macroglobulin 'fast' forms inhibit superoxide production by activated macrophages

Journal Article

Mouse peritoneal macrophages activated by bacillus Calmette-Guerin (BCG) were incubated with human α2-macroglobulin converted to its 'fast' form with either trypsin or methylamine before being stimulated with phorbol myrystate acetate. Both α2-macroglobulin-trypsin and α2-macroglobulin-methylamine inhibited macrophage production of superoxide anion (O2-) while native α2-macroglobulin had little effect except at high concentration. The α2-macroglobulin 'fast' forms, which bind with a Kd of about 8 nM, inhibited 50% generation of O2-(ID50) at a concentration of 7 nM while α2-macroglobulin inhibited O2- production with an ID50 of 141 nM. The 'fast' forms of α2-macroglobulin may play a role in the feedback regulation of inflammatory reactions. © 1983.

Duke Authors

Cited Authors

  • Hoffman, M; Feldman, SR; Pizzo, SV

Published Date

  • 1983

Published In

Volume / Issue

  • 760 / 3

Start / End Page

  • 421 - 423

International Standard Serial Number (ISSN)

  • 0304-4165