Changes in oxygen consumption and phagocytosis in rat pulmonary macrophages related to animal maturation


Journal Article

Differences in selected metabolic and functional parameters of pulmonary macrophages have been found in cells obtained from rats between birth and 40 days of age. Oxygen consumption ranged from 0.6-0.8 nM/106 viable macrophages/min in the pulmonary macrophages of rats from birth to 20 days of age whereas it was 1.4-1.5 nM/106 viable macrophages/min in the pulmonary macrophages of rats 40 days of age and older. One functional test of phagocytosis which measured the combined effects of bacterial ingest ion and killing by macrophages showed that after identical treatment and exposure conditions macrophages from rats less than 15 days of age contained significantly greater numbers of live intracellular colony forming units of Staphy-lococcus aureus than did macrophages from adult rats. A second test of function in which the number of killed Saccharomyces cerevisiae ingested by macrophages was also compared on the basis of animal age indicated that the number of killed yeast ingested was higher in macrophages from 10-day-old rats than macrophages from adults. This ingestion component of phagocytosis was markedly inhibited in the macrophages of adult rats by 10-3 M KCN. In contrast, no statistically significant inhibition of yeast ingestion by KCN was observed in macrophages from 10-day-old rats. This age-dependent differential inhibition by KCN of yeast ingestion suggests that pulmonary macrophages from adult rats depend to a much greater extent upon oxidative phos-phorylation as an energy source than do macrophages from 10-day-old rats. These observations support the conclusion that important age-related biochemical and functional changes occur in the macrophages of rat lungs during animal maturation. © 1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Full Text

Duke Authors

Cited Authors

  • Hoffman, M; Autor, AP

Published Date

  • January 1, 1980

Published In

Volume / Issue

  • 1 / 4

Start / End Page

  • 313 - 322

International Standard Serial Number (ISSN)

  • 0190-2148

Digital Object Identifier (DOI)

  • 10.3109/01902148009069651

Citation Source

  • Scopus