Induction of c-fos expression in the rostral medulla of rats following electroacupuncture stimulation.

Journal Article (Journal Article)

The present study was designed to examine the induction of c-fos mRNA and Fos-like protein in the rostral medulla of awake rats following electroacupuncture (EA) stimulation, using in situ hybridization and immunohistochemical method. Rats were subjected to EA stimulation (100 Hz, 1-2-3 mA) for 30 min by inserting two needles into acupoints Zusanli (S36) and Sanyingjiao (Sp6) of each hind leg. Animals were sacrificed 1h and 2h after EA for in situ hybridization and immunohistochemical study, respectively. c-fos mRNA or Fos-like-immunoreactivity (FLI) neurons were densely distributed in the rostral ventromedial medulla (RVM), including raphe magnus nucleus, gigantocellular reticular nucleus, gigantocellular reticular nucleus alpha, as well as intermediate reticular nucleus. Neurons in the solitary tract nucleus also showed c-fos expression. The results suggest that the RVM may be an important region for mediating EA effect.

Full Text

Duke Authors

Cited Authors

  • Ji, RR; Zhang, ZW; Zhou, Y; Zhang, Q; Han, JS

Published Date

  • October 1993

Published In

Volume / Issue

  • 72 / 3-4

Start / End Page

  • 183 - 191

PubMed ID

  • 8138374

International Standard Serial Number (ISSN)

  • 0020-7454

Digital Object Identifier (DOI)

  • 10.3109/00207459309024107


  • eng

Conference Location

  • England