The movement characteristics of rabbit spermatozoa before and after activation

Journal Article (Journal Article)

Spermatozoa were flushed with mineral oil from the lower isthmus of the rabbit oviduct at four hours postcoitus (pc) and 11 hours pc. Videotapes were made of sperm behavior in the native isthmic fluid and after dilution of the fluid with culture medium. The tapes showed that, initially, spermatozoa in the native isthmic fluid were virtually immotile, but immediately resumed movement on contact with the culture medium. Isthmic sperm motility then evolved over a five‐ to 10‐minute interval into the characteristic biphasic pattern of activated movement. Cine films of isthmic spermatozoa taken with a high‐speed camera were analyzed to determine flagellar beat frequency, maximum flagellar curvature, and swimming velocity. Progressiveness ratios and hydrodynamic power outputs were then calculated for individual spermatozoa. Two phases of activated sperm movement, a whiplash phase and a progressive phase, were identified and characterized. The power output of activated spermatozoa increased twentyfold in comparison with the preactivated state. The power output of activated spermatozoa did not differ between the two phases of activated movement. Copyright © 1981 Alan R. Liss, Inc.

Full Text

Duke Authors

Cited Authors

  • Johnson, LL; Katz, DF; Overstreet, JW

Published Date

  • January 1, 1981

Published In

Volume / Issue

  • 4 / 4

Start / End Page

  • 275 - 282

Electronic International Standard Serial Number (EISSN)

  • 1554-3919

International Standard Serial Number (ISSN)

  • 0148-7280

Digital Object Identifier (DOI)

  • 10.1002/mrd.1120040403

Citation Source

  • Scopus