Molecular analysis of the 60-kDa human Ro ribonucleoprotein.
Journal Article (Journal Article)
Ro, or Sjogren syndrome type A (SS-A), antigen is the most prevalent of the human systemic autoimmune specificities and exists as an inabundant ribonucleoprotein complex (RNP) composed of a 60,649-Da protein, as defined here by cDNA cloning, and the human Y RNAs. The recombinant 60-kDa Ro protein and human Y1 RNA were reconstituted in vitro, and the binding was enhanced by divalent cations. A region of the Ro amino acid sequence revealed a resemblance to the RNP consensus motif found in most RNA-binding proteins. In addition, Ro contained a potential "zinc-binding finger" motif that was distinct from the RNP consensus region and that may participate in the interaction with human Y RNAs or with other proteins. The recombinant Ro fusion protein also proved useful for the detection of autoantibodies in the sera of patients with autoimmune disorders. Possible functions of the Ro RNPs and their relationship to RNA polymerase III transcription are discussed.
Full Text
Duke Authors
Cited Authors
- Deutscher, SL; Harley, JB; Keene, JD
Published Date
- December 1988
Published In
Volume / Issue
- 85 / 24
Start / End Page
- 9479 - 9483
PubMed ID
- 3200833
Pubmed Central ID
- PMC282776
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.85.24.9479
Language
- eng
Conference Location
- United States