RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome.

Journal Article (Journal Article)

The 3' end of the genomic RNA of Newcastle disease virus (NDV) has been sequenced and the leader RNA defined. Using hybridization to a 3'-end-labeled genome, leader RNA species from in vitro transcription reactions and from infected cell extracts were found to be 47 and 53 nucleotides long. In addition, the start site of the 3'-proximal mRNA was determined by sequence analysis of in vitro [beta-82P]GTP-labeled transcription products. The genomic sequence extending beyond the leader region demonstrated an open reading frame for at least 42 amino acids and probably represents the amino terminus of the nucleocapsid protein (NP). The terminal 8 nucleotides of the NDV genome were identical to those of measles virus and Sendai virus while the sequence of the distal half of the leader region was more similar to that of vesicular stomatitis virus. These data argue for strong evolutionary relatedness between the paramyxovirus and rhabdovirus groups.

Full Text

Duke Authors

Cited Authors

  • Kurilla, MG; Stone, HO; Keene, JD

Published Date

  • September 1985

Published In

Volume / Issue

  • 145 / 2

Start / End Page

  • 203 - 212

PubMed ID

  • 4024452

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/0042-6822(85)90154-0


  • eng

Conference Location

  • United States