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The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.

Publication ,  Journal Article
Kurilla, MG; Keene, JD
Published in: Cell
October 1983

The leader RNA transcript of vesicular stomatitis virus (VSV) has been immunoprecipitated from infected BHK cell extracts by anti-La specific sera from patients with systemic lupus erythematosus (SLE). This association was specific as lupus anti-sera with other specificities failed to precipitate leader RNA. The amount of leader RNA associated with the La antigen peaked 4 hr post infection and then declined. Leader RNA complexed with viral nucleocapsid proteins increased at a slower rate but eventually predominated 6 hr post infection. By 16 hr all of the leader RNA was associated with nucleocapsid proteins. Although a significant portion of the leader RNA was present in isolated nuclei 4 hr post infection, all of the leader RNA outside the nucleus was bound to La protein. Leader RNA is the first non-RNA polymerase III product found to associate with the La protein. The proposed function of the leader-La complex in VSV transcription and replication and in viral cytopathology is discussed.

Duke Scholars

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 1983

Volume

34

Issue

3

Start / End Page

837 / 845

Location

United States

Related Subject Headings

  • Virus Replication
  • Viral Proteins
  • Vesicular stomatitis Indiana virus
  • Subcellular Fractions
  • RNA, Viral
  • Lupus Erythematosus, Systemic
  • Developmental Biology
  • Cross Reactions
  • Cricetinae
  • Base Sequence
 

Citation

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Kurilla, M. G., & Keene, J. D. (1983). The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies. Cell, 34(3), 837–845. https://doi.org/10.1016/0092-8674(83)90541-x
Kurilla, M. G., and J. D. Keene. “The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.Cell 34, no. 3 (October 1983): 837–45. https://doi.org/10.1016/0092-8674(83)90541-x.
Kurilla, M. G., and J. D. Keene. “The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.Cell, vol. 34, no. 3, Oct. 1983, pp. 837–45. Pubmed, doi:10.1016/0092-8674(83)90541-x.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 1983

Volume

34

Issue

3

Start / End Page

837 / 845

Location

United States

Related Subject Headings

  • Virus Replication
  • Viral Proteins
  • Vesicular stomatitis Indiana virus
  • Subcellular Fractions
  • RNA, Viral
  • Lupus Erythematosus, Systemic
  • Developmental Biology
  • Cross Reactions
  • Cricetinae
  • Base Sequence