The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.

Published

Journal Article

The leader RNA transcript of vesicular stomatitis virus (VSV) has been immunoprecipitated from infected BHK cell extracts by anti-La specific sera from patients with systemic lupus erythematosus (SLE). This association was specific as lupus anti-sera with other specificities failed to precipitate leader RNA. The amount of leader RNA associated with the La antigen peaked 4 hr post infection and then declined. Leader RNA complexed with viral nucleocapsid proteins increased at a slower rate but eventually predominated 6 hr post infection. By 16 hr all of the leader RNA was associated with nucleocapsid proteins. Although a significant portion of the leader RNA was present in isolated nuclei 4 hr post infection, all of the leader RNA outside the nucleus was bound to La protein. Leader RNA is the first non-RNA polymerase III product found to associate with the La protein. The proposed function of the leader-La complex in VSV transcription and replication and in viral cytopathology is discussed.

Full Text

Duke Authors

Cited Authors

  • Kurilla, MG; Keene, JD

Published Date

  • October 1983

Published In

Volume / Issue

  • 34 / 3

Start / End Page

  • 837 - 845

PubMed ID

  • 6313210

Pubmed Central ID

  • 6313210

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(83)90541-x

Language

  • eng