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The complete sequence of a unique RNA species synthesized by a DI particle of VSV.

Publication ,  Journal Article
Schubert, M; Keene, JD; Lazzarini, RA; Emerson, SU
Published in: Cell
September 1978

The 2S RNA synthesized in vitro by the RNA polymerase of a defective interfering (DI) particle of vesicular stomatitis virus was labeled at its 3' terminus with 32P-cytidine 3', 5' bisphosphate and RNA ligase. Analysis of the labeled RNA showed that it was a family of RNAs of different length but all sharing the same 5' terminal sequence. The largest labeled RNA was purified by gel electrophoresis, and the sequence of 41 of its 46 nucleotides was determined by rapid RNA sequencing methods. The assignment of the remaining 5 nucleotides was made on the basis of an analysis of one of the smaller RNAs and published data. A new approach in RNA sequencing based on the identification of 3' terminal nucleotides of rna fragments originally present in the DI product or generated during the ligation reaction confirmed most of the sequence. The complete sequence of this 46 nucleotide long plus-sense RNA is: ppACGAAGACCACAAAACCAGAUAAAAAA UAAAAACCACAAGAGGGUC-OH. This RNA anneals to the RNA of the DI particle from which it was synthesized, indicating that its synthesis is template-specified. At least the first 17 and possibly all of the nucleotides are also complementary to sequences at the 3' end of two other VSV DI particles which were derived independently and whose genomes differ significantly in length. These data suggest a common 3' terminal sequence among all VSV DI particles which contain part of the Lgene region of the parental genome.

Duke Scholars

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1978

Volume

15

Issue

1

Start / End Page

103 / 112

Location

United States

Related Subject Headings

  • Virus Replication
  • Vesicular stomatitis Indiana virus
  • Transcription, Genetic
  • Templates, Genetic
  • RNA, Viral
  • Oligoribonucleotides
  • Genes, Viral
  • Developmental Biology
  • Defective Viruses
  • Base Sequence
 

Citation

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Schubert, M., Keene, J. D., Lazzarini, R. A., & Emerson, S. U. (1978). The complete sequence of a unique RNA species synthesized by a DI particle of VSV. Cell, 15(1), 103–112. https://doi.org/10.1016/0092-8674(78)90086-7
Schubert, M., J. D. Keene, R. A. Lazzarini, and S. U. Emerson. “The complete sequence of a unique RNA species synthesized by a DI particle of VSV.Cell 15, no. 1 (September 1978): 103–12. https://doi.org/10.1016/0092-8674(78)90086-7.
Schubert M, Keene JD, Lazzarini RA, Emerson SU. The complete sequence of a unique RNA species synthesized by a DI particle of VSV. Cell. 1978 Sep;15(1):103–12.
Schubert, M., et al. “The complete sequence of a unique RNA species synthesized by a DI particle of VSV.Cell, vol. 15, no. 1, Sept. 1978, pp. 103–12. Pubmed, doi:10.1016/0092-8674(78)90086-7.
Schubert M, Keene JD, Lazzarini RA, Emerson SU. The complete sequence of a unique RNA species synthesized by a DI particle of VSV. Cell. 1978 Sep;15(1):103–112.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1978

Volume

15

Issue

1

Start / End Page

103 / 112

Location

United States

Related Subject Headings

  • Virus Replication
  • Vesicular stomatitis Indiana virus
  • Transcription, Genetic
  • Templates, Genetic
  • RNA, Viral
  • Oligoribonucleotides
  • Genes, Viral
  • Developmental Biology
  • Defective Viruses
  • Base Sequence