Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication.
Published
Journal Article
Eukaryotic cells possess overlapping mechanisms to ensure that DNA replication is restricted to the S phase of the cell cycle. The levels of hOrc1p, the largest subunit of the human origin recognition complex, vary during the cell division cycle. In rapidly proliferating cells, hOrc1p is expressed and targeted to chromatin as cells exit mitosis and prereplicative complexes are formed. Later, as cyclin A accumulates and cells enter S phase, hOrc1p is ubiquitinated on chromatin and then degraded. hOrc1p destruction occurs through the proteasome and is signaled in part by the SCF(Skp2) ubiquitin-ligase complex. Other hORC subunits are stable throughout the cell cycle. The regulation of hOrc1p may be an important mechanism in maintaining the ploidy in human cells.
Full Text
Duke Authors
Cited Authors
- Méndez, J; Zou-Yang, XH; Kim, S-Y; Hidaka, M; Tansey, WP; Stillman, B
Published Date
- March 2002
Published In
Volume / Issue
- 9 / 3
Start / End Page
- 481 - 491
PubMed ID
- 11931757
Pubmed Central ID
- 11931757
International Standard Serial Number (ISSN)
- 1097-2765
Digital Object Identifier (DOI)
- 10.1016/s1097-2765(02)00467-7
Language
- eng
Conference Location
- United States