Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication.

Published

Journal Article

Eukaryotic cells possess overlapping mechanisms to ensure that DNA replication is restricted to the S phase of the cell cycle. The levels of hOrc1p, the largest subunit of the human origin recognition complex, vary during the cell division cycle. In rapidly proliferating cells, hOrc1p is expressed and targeted to chromatin as cells exit mitosis and prereplicative complexes are formed. Later, as cyclin A accumulates and cells enter S phase, hOrc1p is ubiquitinated on chromatin and then degraded. hOrc1p destruction occurs through the proteasome and is signaled in part by the SCF(Skp2) ubiquitin-ligase complex. Other hORC subunits are stable throughout the cell cycle. The regulation of hOrc1p may be an important mechanism in maintaining the ploidy in human cells.

Full Text

Duke Authors

Cited Authors

  • Méndez, J; Zou-Yang, XH; Kim, S-Y; Hidaka, M; Tansey, WP; Stillman, B

Published Date

  • March 2002

Published In

Volume / Issue

  • 9 / 3

Start / End Page

  • 481 - 491

PubMed ID

  • 11931757

Pubmed Central ID

  • 11931757

International Standard Serial Number (ISSN)

  • 1097-2765

Language

  • eng

Conference Location

  • United States