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Polymerase chain reaction amplification and in situ hybridization for the detection of human B-lymphotropic virus.

Publication ,  Journal Article
Buchbinder, A; Josephs, SF; Ablashi, D; Salahuddin, SZ; Klotman, ME; Manak, M; Krueger, GR; Wong-Staal, F; Gallo, RC
Published in: J Virol Methods
September 1988

Polymerase chain reaction amplification (PCR) is a recently described technique that allows for the amplification of a given sequence of DNA. It can be used to reliably amplify sequences of up to 3 kb within hours. The amplified sequence can then be recognized by hybridization with a specific probe after transfer onto nitrocellulose or nylon paper. We used PCR to recognize human B-lymphotropic virus (HBLV or HHV-6) specific sequences in various tumors as well as in the blood of patients with AIDS. Sixty-three specimens of DNA extracted from peripheral blood of patients with AIDS as well as DNA extracted from various lymphoproliferative disorders were analysed; 52 out of 63 (83%) patients with AIDS were found to have amplification of the HHV-6 specific sequence; 2 out of the 63 (3%) had equivocal amplification and 9 (14%) were found to be negative. Twenty out of 23 tumors were found to have amplified HBLV-specific sequences. Only one of these tumors was positive by Southern hybridization on restriction enzyme digested genomic DNA. In situ hybridization of clinical specimens using radiolabelled RNA probes or hapten-labelled DNA probes was used to detect the presence of HBLV in tumors. Three tumors of B cell origin were found to be positive for HBLV.

Duke Scholars

Published In

J Virol Methods

DOI

ISSN

0166-0934

Publication Date

September 1988

Volume

21

Issue

1-4

Start / End Page

191 / 197

Location

Netherlands

Related Subject Headings

  • Virology
  • RNA, Viral
  • RNA Probes
  • Nucleic Acid Hybridization
  • Lymphoproliferative Disorders
  • Humans
  • Herpesviridae
  • Gene Amplification
  • DNA, Viral
  • DNA Probes
 

Citation

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MLA
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Buchbinder, A., Josephs, S. F., Ablashi, D., Salahuddin, S. Z., Klotman, M. E., Manak, M., … Gallo, R. C. (1988). Polymerase chain reaction amplification and in situ hybridization for the detection of human B-lymphotropic virus. J Virol Methods, 21(1–4), 191–197. https://doi.org/10.1016/0166-0934(88)90065-1
Buchbinder, A., S. F. Josephs, D. Ablashi, S. Z. Salahuddin, M. E. Klotman, M. Manak, G. R. Krueger, F. Wong-Staal, and R. C. Gallo. “Polymerase chain reaction amplification and in situ hybridization for the detection of human B-lymphotropic virus.J Virol Methods 21, no. 1–4 (September 1988): 191–97. https://doi.org/10.1016/0166-0934(88)90065-1.
Buchbinder A, Josephs SF, Ablashi D, Salahuddin SZ, Klotman ME, Manak M, et al. Polymerase chain reaction amplification and in situ hybridization for the detection of human B-lymphotropic virus. J Virol Methods. 1988 Sep;21(1–4):191–7.
Buchbinder, A., et al. “Polymerase chain reaction amplification and in situ hybridization for the detection of human B-lymphotropic virus.J Virol Methods, vol. 21, no. 1–4, Sept. 1988, pp. 191–97. Pubmed, doi:10.1016/0166-0934(88)90065-1.
Buchbinder A, Josephs SF, Ablashi D, Salahuddin SZ, Klotman ME, Manak M, Krueger GR, Wong-Staal F, Gallo RC. Polymerase chain reaction amplification and in situ hybridization for the detection of human B-lymphotropic virus. J Virol Methods. 1988 Sep;21(1–4):191–197.
Journal cover image

Published In

J Virol Methods

DOI

ISSN

0166-0934

Publication Date

September 1988

Volume

21

Issue

1-4

Start / End Page

191 / 197

Location

Netherlands

Related Subject Headings

  • Virology
  • RNA, Viral
  • RNA Probes
  • Nucleic Acid Hybridization
  • Lymphoproliferative Disorders
  • Humans
  • Herpesviridae
  • Gene Amplification
  • DNA, Viral
  • DNA Probes