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Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism.

Publication ,  Journal Article
Phelps, WC; Bagchi, S; Barnes, JA; Raychaudhuri, P; Kraus, V; Münger, K; Howley, PM; Nevins, JR
Published in: J Virol
December 1991

The human papillomavirus E7 gene product is an oncoprotein with properties similar to those of the adenovirus E1A proteins. The human papillomavirus E7 proteins possess substantial amino acid sequence similarity to portions of conserved regions 1 and 2 of E1A, and the human papillomavirus type 16 E7 protein trans-activates the adenovirus E2 early promoter. Analysis of point mutations in the E2 promoter indicated that the E2F recognition sites were critical to E7 stimulation. In contrast to the activation of the E2 promoter, E7 could not trans-activate various other E1A-inducible promoters. Although the promoter specificity for E7 differs from that of 13S E1A trans activation, it is very similar to activation by the E1A 12S product. Moreover, analysis of the E7 protein has suggested that amino acid sequences critical for trans activation include those shared with E1A within conserved region 2. Biochemical studies demonstrate that the E7 protein, like the 12S E1A product, can alter the interaction of cellular factors with the E2F transcription factor. We therefore conclude that E7 trans activation is functionally related to that mediated by the 12S E1A product.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

December 1991

Volume

65

Issue

12

Start / End Page

6922 / 6930

Location

United States

Related Subject Headings

  • Virology
  • Transcriptional Activation
  • Transcription Factors
  • Sequence Homology, Nucleic Acid
  • Recombinant Fusion Proteins
  • Protein-Tyrosine Kinases
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Papillomavirus E7 Proteins
  • Oncogenes
 

Citation

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Phelps, W. C., Bagchi, S., Barnes, J. A., Raychaudhuri, P., Kraus, V., Münger, K., … Nevins, J. R. (1991). Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism. J Virol, 65(12), 6922–6930. https://doi.org/10.1128/JVI.65.12.6922-6930.1991
Phelps, W. C., S. Bagchi, J. A. Barnes, P. Raychaudhuri, V. Kraus, K. Münger, P. M. Howley, and J. R. Nevins. “Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism.J Virol 65, no. 12 (December 1991): 6922–30. https://doi.org/10.1128/JVI.65.12.6922-6930.1991.
Phelps WC, Bagchi S, Barnes JA, Raychaudhuri P, Kraus V, Münger K, et al. Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism. J Virol. 1991 Dec;65(12):6922–30.
Phelps, W. C., et al. “Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism.J Virol, vol. 65, no. 12, Dec. 1991, pp. 6922–30. Pubmed, doi:10.1128/JVI.65.12.6922-6930.1991.
Phelps WC, Bagchi S, Barnes JA, Raychaudhuri P, Kraus V, Münger K, Howley PM, Nevins JR. Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism. J Virol. 1991 Dec;65(12):6922–6930.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

December 1991

Volume

65

Issue

12

Start / End Page

6922 / 6930

Location

United States

Related Subject Headings

  • Virology
  • Transcriptional Activation
  • Transcription Factors
  • Sequence Homology, Nucleic Acid
  • Recombinant Fusion Proteins
  • Protein-Tyrosine Kinases
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Papillomavirus E7 Proteins
  • Oncogenes