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Induction and maintenance of increased VEGF protein by chronic motor nerve stimulation in skeletal muscle

Publication ,  Journal Article
Annex, BH; Torgan, CE; Lin, P; Taylor, DA; Thompson, MA; Peters, KG; Kraus, WE
Published in: American Journal of Physiology - Heart and Circulatory Physiology
March 1, 1998

Vascular endothelial growth factor (VEGF) causes endothelial cell proliferation in vitro and angiogenesis in vivo. Glycolytic skeletal muscles have a lower capillary density than oxidative muscles but can increase their capillary density and convert to a more oxidative phenotype when subject to chronic motor nerve stimulation (CMNS). We used Western analysis and immunohistochemical techniques to examine VEGF protein in a rabbit CMNS model of glycolytic skeletal muscle and in muscles with innate glycolytic versus oxidative phenotypes. VEGF protein per gram of total protein was increased in stimulated vs. control muscles 2.9 ± 1.0, 3.6 ± 1.3, 3.1 ± 0.5, 4.4 ± 1.6, and 2.7 ± 0.3 times after 3 (n = 4), 5 (n = 2), 10 (n = 3), 21 (n = 3), and 56 (n = 2) days, respectively. VEGF protein was increased 3.1 ± 0.5 times (P < 0.005) before (3, 5, and 10 days) and remained elevated 3.7 ± 1.0 times (P < 0.05) after (21 and 56 days) the transition to an oxidative phenotype. By immunohistochemistry, VEGF protein was found primarily in the matrix between stimulated muscle fibers but not in the myocytes. In addition, VEGF protein was consistently lower in innate glycolytic compared with oxidative muscles. These findings suggest that VEGF plays a role in the alteration and maintenance of vascular density in mammalian skeletal muscles. Copyright © 1998 the American Physiological Society.

Duke Scholars

Published In

American Journal of Physiology - Heart and Circulatory Physiology

ISSN

0363-6135

Publication Date

March 1, 1998

Volume

43

Issue

3

Related Subject Headings

  • Cardiovascular System & Hematology
  • 3208 Medical physiology
  • 3201 Cardiovascular medicine and haematology
  • 1116 Medical Physiology
  • 0606 Physiology
 

Citation

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Annex, B. H., Torgan, C. E., Lin, P., Taylor, D. A., Thompson, M. A., Peters, K. G., & Kraus, W. E. (1998). Induction and maintenance of increased VEGF protein by chronic motor nerve stimulation in skeletal muscle. American Journal of Physiology - Heart and Circulatory Physiology, 43(3).
Annex, B. H., C. E. Torgan, P. Lin, D. A. Taylor, M. A. Thompson, K. G. Peters, and W. E. Kraus. “Induction and maintenance of increased VEGF protein by chronic motor nerve stimulation in skeletal muscle.” American Journal of Physiology - Heart and Circulatory Physiology 43, no. 3 (March 1, 1998).
Annex BH, Torgan CE, Lin P, Taylor DA, Thompson MA, Peters KG, et al. Induction and maintenance of increased VEGF protein by chronic motor nerve stimulation in skeletal muscle. American Journal of Physiology - Heart and Circulatory Physiology. 1998 Mar 1;43(3).
Annex, B. H., et al. “Induction and maintenance of increased VEGF protein by chronic motor nerve stimulation in skeletal muscle.” American Journal of Physiology - Heart and Circulatory Physiology, vol. 43, no. 3, Mar. 1998.
Annex BH, Torgan CE, Lin P, Taylor DA, Thompson MA, Peters KG, Kraus WE. Induction and maintenance of increased VEGF protein by chronic motor nerve stimulation in skeletal muscle. American Journal of Physiology - Heart and Circulatory Physiology. 1998 Mar 1;43(3).

Published In

American Journal of Physiology - Heart and Circulatory Physiology

ISSN

0363-6135

Publication Date

March 1, 1998

Volume

43

Issue

3

Related Subject Headings

  • Cardiovascular System & Hematology
  • 3208 Medical physiology
  • 3201 Cardiovascular medicine and haematology
  • 1116 Medical Physiology
  • 0606 Physiology