Skip to main content
Journal cover image

The inoculum effect and band-pass bacterial response to periodic antibiotic treatment.

Publication ,  Journal Article
Tan, C; Smith, RP; Srimani, JK; Riccione, KA; Prasada, S; Kuehn, M; You, L
Published in: Mol Syst Biol
2012

The inoculum effect (IE) refers to the decreasing efficacy of an antibiotic with increasing bacterial density. It represents a unique strategy of antibiotic tolerance and it can complicate design of effective antibiotic treatment of bacterial infections. To gain insight into this phenomenon, we have analyzed responses of a lab strain of Escherichia coli to antibiotics that target the ribosome. We show that the IE can be explained by bistable inhibition of bacterial growth. A critical requirement for this bistability is sufficiently fast degradation of ribosomes, which can result from antibiotic-induced heat-shock response. Furthermore, antibiotics that elicit the IE can lead to 'band-pass' response of bacterial growth to periodic antibiotic treatment: the treatment efficacy drastically diminishes at intermediate frequencies of treatment. Our proposed mechanism for the IE may be generally applicable to other bacterial species treated with antibiotics targeting the ribosomes.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Mol Syst Biol

DOI

EISSN

1744-4292

Publication Date

2012

Volume

8

Start / End Page

617

Location

England

Related Subject Headings

  • Salmonella typhimurium
  • Ribosomes
  • Proteolysis
  • Models, Biological
  • Microbial Sensitivity Tests
  • Kinetics
  • Kanamycin
  • Heat-Shock Response
  • Escherichia coli
  • Colony Count, Microbial
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tan, C., Smith, R. P., Srimani, J. K., Riccione, K. A., Prasada, S., Kuehn, M., & You, L. (2012). The inoculum effect and band-pass bacterial response to periodic antibiotic treatment. Mol Syst Biol, 8, 617. https://doi.org/10.1038/msb.2012.49
Tan, Cheemeng, Robert Phillip Smith, Jaydeep K. Srimani, Katherine A. Riccione, Sameer Prasada, Meta Kuehn, and Lingchong You. “The inoculum effect and band-pass bacterial response to periodic antibiotic treatment.Mol Syst Biol 8 (2012): 617. https://doi.org/10.1038/msb.2012.49.
Tan C, Smith RP, Srimani JK, Riccione KA, Prasada S, Kuehn M, et al. The inoculum effect and band-pass bacterial response to periodic antibiotic treatment. Mol Syst Biol. 2012;8:617.
Tan, Cheemeng, et al. “The inoculum effect and band-pass bacterial response to periodic antibiotic treatment.Mol Syst Biol, vol. 8, 2012, p. 617. Pubmed, doi:10.1038/msb.2012.49.
Tan C, Smith RP, Srimani JK, Riccione KA, Prasada S, Kuehn M, You L. The inoculum effect and band-pass bacterial response to periodic antibiotic treatment. Mol Syst Biol. 2012;8:617.
Journal cover image

Published In

Mol Syst Biol

DOI

EISSN

1744-4292

Publication Date

2012

Volume

8

Start / End Page

617

Location

England

Related Subject Headings

  • Salmonella typhimurium
  • Ribosomes
  • Proteolysis
  • Models, Biological
  • Microbial Sensitivity Tests
  • Kinetics
  • Kanamycin
  • Heat-Shock Response
  • Escherichia coli
  • Colony Count, Microbial