Bxz1, a new generalized transducing phage for mycobacteria.

Journal Article (Journal Article)

We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae. Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10(-8) to 10(-6) per plaque forming unit between strains of M. smegmatis. We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene.

Full Text

Duke Authors

Cited Authors

  • Lee, S; Kriakov, J; Vilcheze, C; Dai, Z; Hatfull, GF; Jacobs, WR

Published Date

  • December 15, 2004

Published In

Volume / Issue

  • 241 / 2

Start / End Page

  • 271 - 276

PubMed ID

  • 15598543

International Standard Serial Number (ISSN)

  • 0378-1097

Digital Object Identifier (DOI)

  • 10.1016/j.femsle.2004.10.032

Language

  • eng

Conference Location

  • England