Characterization of a major cluster of nif, fix, and associated genes in a sugarcane endophyte, Acetobacter diazotrophicus.


Journal Article

A major 30.5-kb cluster of nif and associated genes of Acetobacter diazotrophicus (syn. Gluconacetobacter diazotrophicus), a nitrogen-fixing endophyte of sugarcane, was sequenced and analyzed. This cluster represents the largest assembly of contiguous nif-fix and associated genes so far characterized in any diazotrophic bacterial species. Northern blots and promoter sequence analysis indicated that the genes are organized into eight transcriptional units. The overall arrangement of genes is most like that of the nif-fix cluster in Azospirillum brasilense, while the individual gene products are more similar to those in species of Rhizobiaceae or in Rhodobacter capsulatus.

Full Text

Duke Authors

Cited Authors

  • Lee, S; Reth, A; Meletzus, D; Sevilla, M; Kennedy, C

Published Date

  • December 2000

Published In

Volume / Issue

  • 182 / 24

Start / End Page

  • 7088 - 7091

PubMed ID

  • 11092875

Pubmed Central ID

  • 11092875

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.182.24.7088-7091.2000


  • eng

Conference Location

  • United States