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Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA

Publication ,  Journal Article
Kanayama, S; Liddle, RA
Published in: American Journal of Physiology - Gastrointestinal and Liver Physiology
January 1, 1990

The gastrointestinal peptides, cholecystokinin (CCK) and somatostatin, are produced by discrete endocrine cells in the mucosa of the small intestine. Although somatostatin may inhibit CCK secretion, the mechanism by which this occurs is unknown. The present study was designed to determine the effect of somatostatin on intestinal CCK and somatostatin mRNA levels. Rats, prepared with indwelling intraduodenal and jugular cannulas, were first fed an elemental diet that did not stimulate CCK release. Next, as a means of stimulating CCK secretion, soybean trypsin inhibitor was added to the diet and perfused intraduodenally at 50 mg/h for 24 h. Trypsin inhibitor caused an 11-fold increase in plasma CCK levels and a 2.4-fold increase in duodenal CCK mRNA levels. Simultaneous intravenous infusion of somatostatin-14 (1-100 μg·kg-1·h-1) reduced trypsin inhibitor-stimulated CCK levels by 50% and lowered trypsin inhibitor-stimulated CCK mRNA levels by 58%. Somatostatin infusion also reduced intestinal somatostatin mRNA levels stimulated by trypsin inhibitor but did not affect basal somatostatin mRNA levels. Duodenal CCK and somatostatin peptide concentrations did not change under any of the experimental conditions. These studies demonstrate that somatostatin reduces duodenal CCK mRNA levels stimulated by diet and suggest that somatostatin itself inhibits duodenal somatostatin gene expression.

Duke Scholars

Published In

American Journal of Physiology - Gastrointestinal and Liver Physiology

ISSN

0002-9513

Publication Date

January 1, 1990

Volume

258

Issue

3 21-3

Related Subject Headings

  • Cardiovascular System & Hematology
 

Citation

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MLA
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Kanayama, S., & Liddle, R. A. (1990). Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA. American Journal of Physiology - Gastrointestinal and Liver Physiology, 258(3 21-3).
Kanayama, S., and R. A. Liddle. “Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA.” American Journal of Physiology - Gastrointestinal and Liver Physiology 258, no. 3 21-3 (January 1, 1990).
Kanayama S, Liddle RA. Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA. American Journal of Physiology - Gastrointestinal and Liver Physiology. 1990 Jan 1;258(3 21-3).
Kanayama, S., and R. A. Liddle. “Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA.” American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 258, no. 3 21-3, Jan. 1990.
Kanayama S, Liddle RA. Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA. American Journal of Physiology - Gastrointestinal and Liver Physiology. 1990 Jan 1;258(3 21-3).

Published In

American Journal of Physiology - Gastrointestinal and Liver Physiology

ISSN

0002-9513

Publication Date

January 1, 1990

Volume

258

Issue

3 21-3

Related Subject Headings

  • Cardiovascular System & Hematology