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Three tightly linked genes encoding human type I keratins: conservation of sequence in the 5'-untranslated leader and 5'-upstream regions of coexpressed keratin genes.

Publication ,  Journal Article
RayChaudhury, A; Marchuk, D; Lindhurst, M; Fuchs, E
Published in: Mol Cell Biol
February 1986

We have isolated and subcloned three separate segments of human DNA which share strong sequence homology with a previously sequenced gene encoding a type I keratin, K14 (50 kilodaltons). Restriction endonuclease mapping has demonstrated that these three genes are tightly linked chromosomally, whereas the K14 gene appears to be separate. As judged by positive hybridization-translation and Northern blot analyses, the central linked gene encodes a keratin, K17, which is expressed in abundance with K14 and two other type I keratins in cultured human epidermal cells. None of these other epidermal keratin mRNAs appears to be generated from the K17 gene through differential splicing of its transcript. The sequence of the K17 gene reveals striking homologies not only with the coding portions and intron positions of the K14 gene, but also with its 5'-noncoding and 5'-upstream sequences. These similarities may provide an important clue in elucidating the molecular mechanisms underlying the coexpression of the two genes.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

February 1986

Volume

6

Issue

2

Start / End Page

539 / 548

Location

United States

Related Subject Headings

  • Skin
  • Protein Sorting Signals
  • Nucleic Acid Hybridization
  • Keratins
  • Humans
  • Genetic Linkage
  • Genes
  • Developmental Biology
  • DNA Restriction Enzymes
  • DNA
 

Citation

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RayChaudhury, A., Marchuk, D., Lindhurst, M., & Fuchs, E. (1986). Three tightly linked genes encoding human type I keratins: conservation of sequence in the 5'-untranslated leader and 5'-upstream regions of coexpressed keratin genes. Mol Cell Biol, 6(2), 539–548. https://doi.org/10.1128/mcb.6.2.539-548.1986
RayChaudhury, A., D. Marchuk, M. Lindhurst, and E. Fuchs. “Three tightly linked genes encoding human type I keratins: conservation of sequence in the 5'-untranslated leader and 5'-upstream regions of coexpressed keratin genes.Mol Cell Biol 6, no. 2 (February 1986): 539–48. https://doi.org/10.1128/mcb.6.2.539-548.1986.
RayChaudhury, A., et al. “Three tightly linked genes encoding human type I keratins: conservation of sequence in the 5'-untranslated leader and 5'-upstream regions of coexpressed keratin genes.Mol Cell Biol, vol. 6, no. 2, Feb. 1986, pp. 539–48. Pubmed, doi:10.1128/mcb.6.2.539-548.1986.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

February 1986

Volume

6

Issue

2

Start / End Page

539 / 548

Location

United States

Related Subject Headings

  • Skin
  • Protein Sorting Signals
  • Nucleic Acid Hybridization
  • Keratins
  • Humans
  • Genetic Linkage
  • Genes
  • Developmental Biology
  • DNA Restriction Enzymes
  • DNA