Antiestrogen can establish nonproductive receptor complexes and alter chromatin structure at target enhancers.

Published

Journal Article

We describe in this report experiments in vivo that demonstrate that antiestrogens promote DNA binding of the estrogen receptor without efficiently inducing transcription. When the receptor is modified to carry a foreign unregulated transactivation domain, transcription can be induced efficiently by both estrogen and antiestrogens. Under apparent saturation conditions, antihormone-receptor complexes binding to responsive enhancer elements elicit only a low level of transcription. In addition, we show that both estrogen and an antiestrogen, nafoxidine, effect very similar alterations in chromatin structure at a responsive promoter. These results indicate that in vivo steroid receptor action can be regulated subsequent to the DNA binding step, by regulating interactions with the target transcriptional machinery. In this regard, antihormones can function by establishing receptor-DNA complexes that are transcriptionally nonproductive.

Full Text

Duke Authors

Cited Authors

  • Pham, TA; Elliston, JF; Nawaz, Z; McDonnell, DP; Tsai, MJ; O'Malley, BW

Published Date

  • April 15, 1991

Published In

Volume / Issue

  • 88 / 8

Start / End Page

  • 3125 - 3129

PubMed ID

  • 2014231

Pubmed Central ID

  • 2014231

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.88.8.3125

Language

  • eng

Conference Location

  • United States