Cloning and expression of full-length cDNA encoding human vitamin D receptor.

Published

Journal Article

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Full Text

Duke Authors

Cited Authors

  • Baker, AR; McDonnell, DP; Hughes, M; Crisp, TM; Mangelsdorf, DJ; Haussler, MR; Pike, JW; Shine, J; O'Malley, BW

Published Date

  • May 1988

Published In

Volume / Issue

  • 85 / 10

Start / End Page

  • 3294 - 3298

PubMed ID

  • 2835767

Pubmed Central ID

  • 2835767

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.85.10.3294

Language

  • eng

Conference Location

  • United States