Kindled seizures result in decreased responsiveness of benzodiazepine receptors to gamma-aminobutyric acid (GABA).
Journal Article
Repeated kindled seizures result in increased numbers of benzodiazepine receptors in fascia dentata membranes. Our previous work localized these receptors to a discrete neuronal population, the dentate granule cells. The present investigation characterizes the regulation of this binding by NaCl, pentobarbital and gamma-aminobutyric acid (GABA). [3H]Flunitrazepam binding in membranes from kindled rats exceeded that from controls by 24%. NaCl increased this binding in membranes prepared from control and kindled animals by roughly equivalent amounts (18 and 14%, respectively). No significant differences were found in the effects of pentobarbital on benzodiazepine binding between kindled and control groups. In contrast, addition of GABA increased benzodiazepine binding by a significantly smaller percentage in the kindled (116 +/- 5%) compared to the control (138 +/- 11%) group. We propose that this altered molecular response to GABA is intimately related to the enhanced GABA-mediated inhibition of granule cells present after kindled seizures.
Full Text
Duke Authors
Cited Authors
- Fanelli, RJ; McNamara, JO
Published Date
- July 1, 1983
Published In
Volume / Issue
- 226 / 1
Start / End Page
- 147 - 150
PubMed ID
- 6306222
Pubmed Central ID
- 6306222
International Standard Serial Number (ISSN)
- 0022-3565
Language
- eng
Conference Location
- United States