Kindled seizures result in decreased responsiveness of benzodiazepine receptors to gamma-aminobutyric acid (GABA).

Published

Journal Article

Repeated kindled seizures result in increased numbers of benzodiazepine receptors in fascia dentata membranes. Our previous work localized these receptors to a discrete neuronal population, the dentate granule cells. The present investigation characterizes the regulation of this binding by NaCl, pentobarbital and gamma-aminobutyric acid (GABA). [3H]Flunitrazepam binding in membranes from kindled rats exceeded that from controls by 24%. NaCl increased this binding in membranes prepared from control and kindled animals by roughly equivalent amounts (18 and 14%, respectively). No significant differences were found in the effects of pentobarbital on benzodiazepine binding between kindled and control groups. In contrast, addition of GABA increased benzodiazepine binding by a significantly smaller percentage in the kindled (116 +/- 5%) compared to the control (138 +/- 11%) group. We propose that this altered molecular response to GABA is intimately related to the enhanced GABA-mediated inhibition of granule cells present after kindled seizures.

Full Text

Duke Authors

Cited Authors

  • Fanelli, RJ; McNamara, JO

Published Date

  • July 1, 1983

Published In

Volume / Issue

  • 226 / 1

Start / End Page

  • 147 - 150

PubMed ID

  • 6306222

Pubmed Central ID

  • 6306222

International Standard Serial Number (ISSN)

  • 0022-3565

Language

  • eng

Conference Location

  • United States