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Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines.

Publication ,  Journal Article
Holmes, J; Clark, S; Modrich, P
Published in: Proc Natl Acad Sci U S A
August 1990

Nuclear extracts derived from HeLa and Drosophila melanogaster KC cell lines have been found to correct single base-base mispairs within open circular DNA heteroduplexes containing a strand-specific, site-specific incision located 808 base pairs from the mismatch. Correction in both extract systems is strand specific, being highly biased to the incised DNA strand. Different mispairs within a homologous set of heteroduplexes were processed with different efficiencies (G.T greater than G.G approximately equal to A.C greater than C.C), and correction was accompanied by mismatch-dependent DNA synthesis localized to the region spanning the mispair and the strand break, thus demonstrating that mismatch recognition is associated with the repair reaction. Correction of each of these heteroduplexes was abolished by aphidicolin but was relatively insensitive to the presence of high concentrations of ddTTP, indicating probable involvement of alpha and/or delta class DNA polymerase(s). These findings suggest that higher eukaryotic cells possess a general, strand-specific mismatch repair system analogous to the Escherichia coli mutHLS and the Streptococcus pneumoniae hexAB pathways, systems that contribute in a major way to the genetic stability of these bacterial species.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

August 1990

Volume

87

Issue

15

Start / End Page

5837 / 5841

Location

United States

Related Subject Headings

  • Restriction Mapping
  • Nucleic Acid Heteroduplexes
  • Molecular Sequence Data
  • Kinetics
  • Humans
  • Hela Cells
  • HeLa Cells
  • Gene Conversion
  • Drosophila melanogaster
  • Diterpenes
 

Citation

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Holmes, J., Clark, S., & Modrich, P. (1990). Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines. Proc Natl Acad Sci U S A, 87(15), 5837–5841. https://doi.org/10.1073/pnas.87.15.5837
Holmes, J., S. Clark, and P. Modrich. “Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines.Proc Natl Acad Sci U S A 87, no. 15 (August 1990): 5837–41. https://doi.org/10.1073/pnas.87.15.5837.
Holmes J, Clark S, Modrich P. Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5837–41.
Holmes, J., et al. “Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines.Proc Natl Acad Sci U S A, vol. 87, no. 15, Aug. 1990, pp. 5837–41. Pubmed, doi:10.1073/pnas.87.15.5837.
Holmes J, Clark S, Modrich P. Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5837–5841.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

August 1990

Volume

87

Issue

15

Start / End Page

5837 / 5841

Location

United States

Related Subject Headings

  • Restriction Mapping
  • Nucleic Acid Heteroduplexes
  • Molecular Sequence Data
  • Kinetics
  • Humans
  • Hela Cells
  • HeLa Cells
  • Gene Conversion
  • Drosophila melanogaster
  • Diterpenes