Gap formation is associated with methyl-directed mismatch correction under conditions of restricted DNA synthesis.

Published

Journal Article

A covalently closed, circular heteroduplex containing a G-T mismatch and a single hemimethylated d(GATC) site is subject to efficient methyl-directed mismatch correction in Escherichia coli extracts when repair DNA synthesis is severely restricted by limiting the concentration of exogenously supplied deoxyribonucleoside-5'-triphosphates or by supplementing reactions with chain-terminating 2',3'-dideoxynucleoside triphosphates. However, repair under these conditions results in formation of a single-strand gap in the region of the molecule containing the mismatch and the d(GATC) site. These findings indicate that repair DNA synthesis required for methyl-directed correction can initiate in the vicinity of the mispair, and they are most consistent with a repair reaction involving 3'----5' excision (or strand displacement) from the d(GATC) site followed by 5'----3' repair DNA synthesis initiating in the vicinity of the mismatch.

Full Text

Duke Authors

Cited Authors

  • Su, SS; Grilley, M; Thresher, R; Griffith, J; Modrich, P

Published Date

  • 1989

Published In

Volume / Issue

  • 31 / 1

Start / End Page

  • 104 - 111

PubMed ID

  • 2687086

Pubmed Central ID

  • 2687086

International Standard Serial Number (ISSN)

  • 0831-2796

Digital Object Identifier (DOI)

  • 10.1139/g89-020

Language

  • eng

Conference Location

  • Canada