DNA mismatch correction in a defined system.
Published
Journal Article
DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.
Full Text
Duke Authors
Cited Authors
- Lahue, RS; Au, KG; Modrich, P
Published Date
- July 14, 1989
Published In
Volume / Issue
- 245 / 4914
Start / End Page
- 160 - 164
PubMed ID
- 2665076
Pubmed Central ID
- 2665076
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.2665076
Language
- eng
Conference Location
- United States