DNA mismatch correction in a defined system.


Journal Article

DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.

Full Text

Duke Authors

Cited Authors

  • Lahue, RS; Au, KG; Modrich, P

Published Date

  • July 14, 1989

Published In

Volume / Issue

  • 245 / 4914

Start / End Page

  • 160 - 164

PubMed ID

  • 2665076

Pubmed Central ID

  • 2665076

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.2665076


  • eng

Conference Location

  • United States