Investigation of the complexes of EcoRI endonuclease with decanucleotides containing canonical and modified recognition sequences using fluorescence and optical detection of magnetic resonance spectroscopy.

Published

Journal Article

The binding of EcoRI endonuclease to the oligonucleotides d(GCGAATTCGC) and d(GCGAA) (5BrdU) (5BrdU) d(CGC) has been investigated to determine whether stacking interactions occur between tryptophan residues and the DNA bases. Fluorescence binding isotherms show that the decamer containing the canonical and that containing the modified recognition sequence bind with comparable affinity. Optically detected magnetic resonance spectra show limited perturbations of the Trp zero-field splitting parameters, which are assigned to electrical field effects. No evidence for Trp stacking interactions has been found.

Full Text

Duke Authors

Cited Authors

  • Jhon, NI; Casas-Finet, JR; Maki, AH; Modrich, P

Published Date

  • February 28, 1988

Published In

Volume / Issue

  • 949 / 2

Start / End Page

  • 189 - 194

PubMed ID

  • 2829965

Pubmed Central ID

  • 2829965

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0167-4781(88)90082-6

Language

  • eng

Conference Location

  • Netherlands